Analysis of the low-molecular weight antioxidants of transgenic plants Nicotiana tabacum under abiotic stress conditions

Authors

  • Kristina V. Pristupa Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus https://orcid.org/0000-0003-1977-4949 (unauthenticated)
  • Tatsiana A. Kukulianskaya Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Elena A. Khramtsova Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

Keywords:

antioxidant system, low-molecular weight antioxidants, acdS-gene, Nicotiana tabacum

Abstract

We conducted a comparative analysis of biochemical parameters of non-transgenic and transgenic Nicotiana tabacum, plants cultivated in heavy metal polluted soils. Transgenic plants had in their genome a bacterial acdS-gene encoding the 1-aminocyclopropane-1-carboxylate deaminase (ACC-deaminase) enzyme. The introduction of elevated concentrations of copper, chromium, and lead ions into the soil promotes induction of the acdS-gene expression and an increase in ACC-deaminase activity in transgenic plants. It was shown that the content of phenolic compounds (flavonoids), ascorbic acid, the total antioxidant activity of plants increased under abiotic stress.

Author Biographies

  • Kristina V. Pristupa, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    postgraduate student at the department of biochemistry, faculty of biology

  • Tatsiana A. Kukulianskaya, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    PhD (biology), docent; associate professor at the department of biochemistry, faculty of biology

  • Elena A. Khramtsova, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    PhD (biology), docent; associate professor at the department of genetics, faculty of biology

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Published

2020-02-18

Issue

No. 1 (2020): Journal of the Belarusian State University. Biology

Section

Biochemistry

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How to Cite

Pristupa, K. V. ., Kukulianskaya, T. A. ., & Khramtsova, E. A. . (2020). Analysis of the low-molecular weight antioxidants of transgenic plants Nicotiana tabacum under abiotic stress conditions. Experimental Biology and Biotechnology, 1, 20-26. https://doi.org/10.33581/2521-1722-2020-1-20-26