Growth inhibition and induction of programmed cell death in the root of He­lianthus annuus L. triggered by nickel ions and nickel-histidine complexes

  • Viera S. Mackievic Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus https://orcid.org/0000-0002-5116-0157
  • Artur A. Shyker Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Siarhej M. Zvanarou Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Kiryl S. Litskevich Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Oksana A. Turovets Palessky Institute of Plant Growing, National Academy of Sciences of Belarus, 2 Škoĺnaja Street, Kryničny 247781, Mazyr District, Homieĺ Region, Belarus
  • Ihar I. Smolich Белорусский государственный университет, пр. Независимости, 4, 220030, г. Минск, Беларусь
  • Anatoly I. Sokolik Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Vadim V. Demidchik Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
DOI: https://doi.org/10.33581/2521-1722-2020-1-11-19

Abstract

Nickel (Ni2+) performs a number of vital functions in plants, but at high concentrations it causes toxic effects and inhibits their growth and development. The problem of nickel contamination has a special place in environmental physiology, since the refuse to use nickel alloys and therefore the reduction of Ni2+ release into the biosphere are practically impossible today. In nature one of the mechanisms of adaptation to the excessive nickel content in the medium is increased synthesis of histidine, which forms chelates with Ni2+. In the present work, for the first time, a detailed analysis of the effects of nickel and nickel-histidine complexes on the growth and development of sunflower roots (Helianthus annuus L.) of Belarusian selection (variety Orion) was carried out. It was established that Ni2+, starting from its level in the medium of 0.3 mmol/L, caused a significant growth inhibition of this agricultural plant. At a nickel concentration in the medium above 3 mmol/L, the growth of sunflower stopped completely. Histidine, introduced together with Ni2+, caused a decrease in the toxicity of this metal for the root system, in some cases shifting by single-order nickel concentrations, causing the same effect. Nickel treatment caused a significant increase in the percentage of cells with programmed cell death symptoms, while histidine inhibited the development of these symptoms. Thus, the concentration dependences of nickel and nickel-histidine complexes effects on root growth and the processes of programmed cell death in the sunflower root were first demonstrated.

Author Biographies

Viera S. Mackievic, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

postgraduate student at the department of plant cell biology and bioengineering, faculty of biology

Artur A. Shyker, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

student at the faculty of biology

Siarhej M. Zvanarou, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

postgraduate student at the department of plant cell biology and bioengineering, faculty of biology

Kiryl S. Litskevich, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

student at the faculty of biology

Oksana A. Turovets, Palessky Institute of Plant Growing, National Academy of Sciences of Belarus, 2 Škoĺnaja Street, Kryničny 247781, Mazyr District, Homieĺ Region, Belarus

senior researcher at the laboratory of oil-seeds

Ihar I. Smolich, Белорусский государственный университет, пр. Независимости, 4, 220030, г. Минск, Беларусь

PhD (biology), docent; head of the department of plant cell biology and bioengineering, faculty of biology

Anatoly I. Sokolik, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

PhD (biology); head of the laboratory of plant physiology and biotechnology, associate professor at the department of plant cell biology and bioengineering, faculty of biology

Vadim V. Demidchik, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

doctor of science (biology), docent; dean of the faculty of biology

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Published
2020-02-24
Keywords: sunflower, root, growth, programmed cell death, heavy metals, nickel, histidine
How to Cite
Mackievic, V. S., Shyker, A. A., Zvanarou, S. M., Litskevich, K. S., Turovets, O. A., Smolich, I. I., Sokolik, A. I., & Demidchik, V. V. (2020). Growth inhibition and induction of programmed cell death in the root of He­lianthus annuus L. triggered by nickel ions and nickel-histidine complexes. Experimental Biology and Biotechnology, 1, 11-19. https://doi.org/10.33581/2521-1722-2020-1-11-19
Issue
No 1 (2020): Journal of the Belarusian State University. Biology
Section
Cell Biology and Physiology

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