Effect of emoxipine on cytotoxicity of peripheral blood mononuclears under cultivation with cytarabine and cyclocytidine

Authors

  • Darya B. Nizheharodava International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus; Belarusian Medical Academy of Postgraduate Education, 3 P. Broŭki Street, 3 building, Minsk 220013, Belarus
  • Marina M. Zafranskaya International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus
  • Eugenii I. Kvasyuk International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus
  • Aliaksei G. Sysa International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus

Keywords:

cytarabine, cyclocytidine, oxidative stress, emoxipine, cytotoxicity
Supporting Agencies
This work was supported by Ministry of Education of the Republic of Belarus and Belarusian Republican Fund for Fundamental Researches (grants No. Х18МВ-019, No. М20МС-043).

Abstract

Taking into account the special role of oxidative stress that increases during cancer chemotherapy, the effect of the antioxidant emoxipine on peripheral blood mononuclears was studied under conditions that simulate the cytotoxic effects of antimetabolites of a number of modified cytidine nucleosides in relation to the tumor cell line K562. Lymphoid cells were also a source for subsequent modelling of the immune response to the cancer. It was found that neither the modified nucleosides themselves nor their combination with emoxipine caused changes in IL-2-stimulated cytotoxicity of lymphoid cells in relation to K562 tumor cell line. A study of the expression of the CD107a marker showed a significant stimulating effect of 1 µmol/L of citarabine on the activation of subpopulations of T-lymphocytes (CD3+ ) and cytotoxic T-lymphocytes (CD3+ CD8+ ).

Author Biographies

  • Darya B. Nizheharodava, International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus; Belarusian Medical Academy of Postgraduate Education, 3 P. Broŭki Street, 3 building, Minsk 220013, Belarus

    PhD (biology), docent; associate professor at the department of immunology, faculty of environmental medicine, International Sakharov Environmental Institute, Belarusian State University; and leading researcher, head of the department of immunology and biomedical technologies, Belarusian Medical Academy of Postgraduate Education

  • Marina M. Zafranskaya, International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus

    doctor of science (medicine), docent; head of the department of immunology, faculty of environmental medicine

  • Eugenii I. Kvasyuk, International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus

    doctor of science (chemistry), full professor; professor at the department of environmental chemistry and biochemistry, faculty of environmental medicine

  • Aliaksei G. Sysa, International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus

    PhD (chemistry), docent; dean of the faculty of environmental medicine

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Published

2021-06-23

Issue

No. 2 (2021): Journal of the Belarusian State University. Biology

Section

Cell Biology and Physiology

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How to Cite

Nizheharodava, D. B., Zafranskaya, M. M., Kvasyuk, E. I., & Sysa, A. G. (2021). Effect of emoxipine on cytotoxicity of peripheral blood mononuclears under cultivation with cytarabine and cyclocytidine. Experimental Biology and Biotechnology, 2, 3-10. https://doi.org/10.33581/2521-1722-2021-2-3-10