Virulent properties of pectobacteria isolated from plant samples on the territory of Belarus

  • Shao Chengyue Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus
  • Anatoli N. Evtushenkov Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus

Abstract

The phytopathogenic bacteria Pectobacterium carotovorum, P. parmentieri and P. brasiliense isolated from plants on the territory of Belarus differed in their ability to infect potato, carrot, onion and Chinese cabbage tissues with artificial infection. Strains of species P. carotovorum and P. brasiliense turned out to be highly virulent. Bacteria P. parmentieri were characterised by low virulence, which was manifested in the low efficiency of infection of onion, carrot, Chinese cabbage plants and reduced macerating and pectate lyase activities.

Author Biographies

Shao Chengyue, Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus

postgraduate student at the department of molecular biology, faculty of biology

Anatoli N. Evtushenkov, Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus

doctor of science (biology), full professor; head of the department of molecular biology, faculty of biology

References

  1. Dye DW. Ataxonomic study of the genus Erwinia. I. The amylovora group. New Zealand Journal of Science. 1968;11(4):590–607.
  2. Adeolu M, Alnajar S, Naushad S, Gupta RS. Genome-based phylogeny and taxonomy of the «Enterobacteriales»: proposal for Enterobacterales ord. nov. divided into the families Enterobacteriaceae, Erwiniaceae fam. nov., Pectobacteriaceae fam. nov., Yersiniaceae fam. nov., Hafniaceae fam. nov., Morganellaceae fam. nov., and Budviciaceae fam. nov. International Journal of Systematic and Evolutionary Microbiology. 2016;66(12):5575–5599. DOI: 10.1099/ijsem.0.001485.
  3. Mansfield J, Genin S, Magori S, Citovsky V, Sriariyanum M, Ronald P, et al. Top 10 plant pathogenic bacteria in molecular plant pathology. Molecular Plant Pathology. 2012;13(6):614–629. DOI: 10.1111/j.1364-3703.2012.00804.x.
  4. Pérombelon MCM, Kelman A. Ecology of the soft rot erwinias. Annual Review of Phytopathology. 1980;18:361–387. DOI: 10.1146/annurev.py.18.090180.002045.
  5. Toth IK, van der Wolf JM, Saddler G, Lojkowska E, Hélias V, Pirhonen M, et al. Dickeya species: an emerging problem for potato production in Europe. Plant Pathology. 2011;60(3):385–399. DOI: 10.1111/j.1365-3059.2011.02427.x.
  6. Cappaert MR, Powelson ML, Franc GD, Harrison MD. Irrigation water as a source of inoculum of soft rot erwinias for aerial stem rot of potatoes. Phytopathology. 1988;78(12):1668–1672. DOI: 10.1094/Phyto-78-1668.
  7. Rossmann S, Dees MW, Perminow J, Meadow R, Brurberg MB. Soft rot Enterobacteriaceae are carried by a large range of insect species in potato fields. Applied and Environmental Microbiology. 2018;84(12):e00281-18. DOI: 10.1128/AEM.00281-18.
  8. Pérombelon MCM. Potato diseases caused by soft rot erwinias: an overview of pathogenesis. Plant Pathology. 2002;51(1):1–12. DOI: 10.1046/j.0032-0862.2001.Shorttitle.doc.x.
  9. Hugouvieux-Cotte-Pattat N, Condemine G, Nasser W, Reverchon S. Regulation of pectinolysis in Erwinia chrysanthemi. Annual Review of Microbiology. 1996;50:213–257. DOI: 10.1146/annurev.micro.50.1.213.
  10. Motyka-Pomagruk A, Zoledowska S, Sledz W, Lojkowska E. The occurrence of bacteria from different species of Pectobacteriaceae on seed potato plantations in Poland. European Journal of Plant Pathology. 2021;159(2):309–325. DOI: 10.1007/s10658-020-02163-x.
  11. Evtushenkov AN, Fomichev YuK. [Pectate lyase activity of the bacteria of the genus Erwinia]. Vestnik BGU. Seriya 2, Khimiya. Biologiya. Geografiya. 1978;2:25–28. Russian.
  12. Komar EI, Shavel’ MI, Pesnyakevich AG. [Identification of the gramnegative pectolytic phythopathogenic bacteria provoking potato diseases in Belarus]. Vestnik BGU. Seriya 2, Khimiya. Biologiya. Geografiya. 2014;2:54–60. Russian.
  13. Shao Chengyue, Gorovik YuN, Sidorova SG, Evtushenkov AN. Identification of pectolytic bacterial species isolated during plant bacteriosis in the Republic of Belarus. Experimental Biology and Biotechnology. 2022;3:64–72. Russian. DOI: 10.33581/2957-5060-2022-3-64-72.
  14. Evtushenkov AN, Shevchik VE, Popova LB, Fomichev YuK. [Purification and properties of two extracellular pectate lyases of the strain Erwinia chrysanthemi ENA49]. Prikladnaya biokhimiya i mikrobiologiya. 1986;22(2):187–192. Russian.
  15. Oulghazi S, Sarfraz S, Zaczek-Moczydłowska MA, Khayi S, Ed-Dra A, Lekbach Y, et al. Pectobacterium brasiliense: genomics, host range and disease management. Microorganisms. 2021;9(1):106. DOI: 10.3390/microorganisms9010106.
  16. Block E. The chemistry of garlic and onions. Scientific American. 1985;252(3):114–119.
  17. de Werra P, Debonneville C, Kellenberger I, Dupuis B. Pathogenicity and relative abundance of Dickeya and Pectobacterium species in Switzerland: an epidemiological dichotomy. Microorganisms. 2021;9(11):2270. DOI: 10.3390/microorganisms9112270.
  18. Meng Xianglong, Chai Ali, Shi Yanxia, Xie Xuewen, Ma Zhanhong, Li Baoju. Emergence of bacterial soft rot in cucumber caused by Pectobacterium carotovorum subsp. brasiliense in China. Plant Disease. 2017;101(2):279–287. DOI: 10.1094/PDIS-05-16-0763-RE.
  19. van der Merwe JJ, Coutinho TA, Korsten L, van der Waals JE. Pectobacterium carotovorum subsp. brasiliensis causing blackleg on potatoes in South Africa. European Journal of Plant Pathology. 2010;126(2):175–185. DOI: 10.1007/s10658-009-9531-2.
  20. Oulghazi S, Moumni M, Khayi S, Robic K, Sarfraz S, Lopez-Roques C, et al. Diversity of Pectobacteriaceae species in potato growing regions in Northern Morocco. Microorganisms. 2020;8(6):895. DOI: 10.3390/microorganisms8060895.
  21. Naas H, Sebaihia M, Orfei B, Rezzonico F, Buonaurio R, Moretti C. Pectobacterium carotovorum subsp. brasiliense and Pectobacterium carotovorum subsp. carotovorum as causal agents of potato soft rot in Algeria. European Journal of Plant Pathology. 2018;151(4):1027–1034. DOI: 10.1007/s10658-018-1438-3.
  22. Onkendi EM, Moleleki LN. Characterization of Pectobacterium carotovorum subsp. carotovorum and brasiliense from diseased potatoes in Kenya. European Journal of Plant Pathology. 2014;139(3):557–566. DOI: 10.1007/s10658-014-0411-z.
  23. Leite LN, de Haan EG, Krijger M, Kastelein P, van der Zouwen PS, van den Bovenkamp GW, et al. First report of potato blackleg caused by Pectobacterium carotovorum subsp. brasiliensis in the Netherlands. New Disease Reports. 2014;29(1):24. DOI: 10.5197/j.2044-0588.2014.029.024.
  24. de Werra P, Bussereau F, Keiser A, Ziegler D. First report of potato blackleg caused by Pectobacterium carotovorum subsp. brasiliense in Switzerland. Plant Disease. 2015;99(4):551. DOI: 10.1094/PDIS-07-14-0742-PDN.
  25. Voronina MV, Kabanova AP, Shneider MM, Korzhenkov AA, Toschakov SV, Miroshnikov KK, et al. First report of Pectobacterium carotovorum subsp. brasiliense causing blackleg and stem rot disease of potato in Russia. Plant Disease. 2019;103(2):364. DOI: 10.1094/PDIS-03-18-0456-PDN.
  26. Khayi S, Cigna J, Chong TM, Quêtu-Laurent A, Chan K-G, Hélias V, et al. Transfer of the potato plant isolates of Pectobacterium wasabiae to Pectobacterium parmentieri sp. nov. International Journal of Systematic and Evolutionary Microbiology. 2016; 66(12):5379–5383. DOI: 10.1099/ijsem.0.001524.
  27. Ngoc Ha VT, Voronina MV, Kabanova AP, Shneider MM, Korzhenkov AA, Toschakov SV, et al. First report of Pectobacterium parmentieri causing stem rot disease of potato in Russia. Plant Disease. 2019;103(1):144. DOI: 10.1094/PDIS-11-17-1829-PDN.
Published
2023-06-25
Keywords: soft rot, virulence, pectate lyase, potato, P. brasiliense, P. parmentieri, P. carotovorum
How to Cite
Chengyue, S., & Evtushenkov, A. N. (2023). Virulent properties of pectobacteria isolated from plant samples on the territory of Belarus. Experimental Biology and Biotechnology, 2, 44-51. Retrieved from https://journals.bsu.by/index.php/biology/article/view/5514
Issue
No 2 (2023): Experimental Biology and Biotechnology
Section
Biotechnology and Microbiology

Copyright (c) 2023 Experimental Biology and Biotechnology

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

The authors who are published in this journal agree to the following:

  1. The authors retain copyright on the work and provide the journal with the right of first publication of the work on condition of license Creative Commons Attribution-NonCommercial. 4.0 International (CC BY-NC 4.0).
  2. The authors retain the right to enter into certain contractual agreements relating to the non-exclusive distribution of the published version of the work (e.g. post it on the institutional repository, publication in the book), with the reference to its original publication in this journal.
  3. The authors have the right to post their work on the Internet (e.g. on the institutional store or personal website) prior to and during the review process, conducted by the journal, as this may lead to a productive discussion and a large number of references to this work. (See The Effect of Open Access.)