Optimisation of regeneration conditions of Norway spruce microplants from highly productive embryogenic line

  • Marina P. Kusenkova Forest Institute, National Academy of Sciences of Belarus, 71 Pralietarskaja Street, Gomiel 246050, Belarus

Abstract

The article presents the results of the study on the influence of nutrient media-composition, including the abscisic acid in different concentrations, on somatic embryo maturation and germination. The callus tissue of Norway spruce highly productive cell line (M2), obtained from seeds of Belarusian origin, was selected as the object of experimental study. Among the tested variants of abscisic acid concentration (15; 30 and 60 mmol/L) during somatic embryo maturation stage using the last two gave a pronounced positive effect. It has been showed that Litvay medium with the halfstrength macrosalts and the addition of activated charcoal (5 g/L) and glutamine (0.5 g/L) is optimal for the somatic embryo germination stage.

Author Biography

Marina P. Kusenkova, Forest Institute, National Academy of Sciences of Belarus, 71 Pralietarskaja Street, Gomiel 246050, Belarus

researcher at the department of genetics, breeding and biotechnology

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Published
2023-12-09
Keywords: somatic embryogenesis, Norway spruce, embryogenic cultures, somatic embryo, regenerant
Supporting Agencies This work was supported by the Belarusian Republican Foundation for Fundamental Research (grant No. B21M-071). The author expresses gratitude to the employees of the department of genetics, breeding and biotechnology, Forest Institute, National Academy of Sciences of Belarus, doctor of science (biology), corresponding member of the National Academy of Sciences of Belarus, full professor V. E. Padutov, PhD (biology) S. I. Ivanovskaya and D. V. Kulagin for help in planning and organising of experimental work and valuable comments on the text of the article.
How to Cite
Kusenkova, M. P. (2023). Optimisation of regeneration conditions of Norway spruce microplants from highly productive embryogenic line. Experimental Biology and Biotechnology, 3, 47-56. Retrieved from https://journals.bsu.by/index.php/biology/article/view/5587
Issue
No 3 (2023): Experimental Biology and Biotechnology
Section
Biotechnology and Microbiology

Copyright (c) 2023 Experimental Biology and Biotechnology

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