Effect of quercetin and its microstructured forms on cell viability and nuclear DNA degradation in human keratinocytes when modelling oxidative stress

  • Alla I. Potapovich Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus
  • Tatyana V. Kostyuk Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus
  • Tatsiana G. Shutava Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, 36 F. Skaryny Street, Minsk 220141, Belarus
  • Vladimir A. Kostyuk Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus

Abstract

Experimental data are presented indicating the possibility of using cumoquinone to model oxidative stress and study its consequences in cultured human keratinocytes of the HaCaT line. It has been shown that cell death, DNA damage and the appearance of atypical DNA comets in keratinocytes exposed to cumoquinone are associated not with its genotoxicity, but mainly with the development of oxidative stress. It has been established that quercetin has a cytoprotective effect and reduces the degree of damage to nuclear DNA under conditions of oxidative stress initiated by cumoquinone. This effect increases significantly when microstructured forms of quercetin are used, which may be due to an increase in its cellular availability.

Author Biographies

Alla I. Potapovich, Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus

PhD (biology), docent; leading researcher at the laboratory of physiology, department of human and animal physiology, faculty of biology

Tatyana V. Kostyuk, Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus

researcher at the laboratory of physiology, department of human and animal physiology, faculty of biology

Tatsiana G. Shutava, Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, 36 F. Skaryny Street, Minsk 220141, Belarus

PhD (chemistry), docent; leading researcher at the laboratory of organic composite materials

Vladimir A. Kostyuk, Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus

doctor of science (chemistry), full professor; head of the laboratory of physiology, department of human and animal physiology, faculty of biology

References

  1. Kostyuk VA, Potapovich AI. Bioradicals and bioantioxidants. Minsk: Belarusian State University; 2004. 179 p. Russian.
  2. Korkina LG, De Luca C, Kostyuk VA, Pastore S. Plant polyphenols and tumors: from mechanisms to therapies, prevention, and protection against toxicity of anti-cancer treatments. Current Medicinal Chemistry. 2009;16(30):3943–3965. DOI: 10.2174/0929 86709789352312.
  3. Zou Y, Qian Y, Rong X, Cao K, McClements DJ, Hu K. Encapsulation of quercetin in biopolymer-coated zein nanoparticles: formation, stability, antioxidant capacity, and bioaccessibility. Food Hydrocolloids. 2021;120:106980. DOI: 10.1016/j.foodhyd. 2021.106980.
  4. O’Brien PJ. Molecular mechanisms of quinone cytotoxicity. Chemico-Biological Interactions. 1991;80(1):1–41. DOI: 10.1016/ 0009-2797(91)90029-7.
  5. Potapovich АI, Ignatovich LV, Kostyuk VA, Shadyro OI. Effect of oxygen level on the cytotoxic effect of doxorubicin and thymoquinone. News of Biomedical Sciences. 2021;21(4):167–172. Russian.
  6. Moore GA, Rossi L, Nicotera P, Orrenius S, O’Brien PJ. Quinone toxicity in hepatocytes: studies on mitochondrial Ca2+ release induced by benzoquinone derivatives. Archives of Biochemistry and Biophysics. 1987;259(2):283–295. DOI: 10.1016/0003-9861(87) 90495-4.
  7. Potapovich A, Kostyuk V. Cell-cell death communication by signals passing through non-aqueous environments. Results in Chemistry. 2021;3:100107. DOI: 10.1016/j.rechem.2021.100107.
  8. Shadyro OI, Edimecheva IP, Glushonok GK, Ostrovskaya NI, Polozov GI, Murase H, et al. Effects of phenolic compounds on reactions involving various organic radicals. Free Radical Research. 2003;37(10):1087–1097. DOI: 10.1080/10715760310001600417.
  9. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research. 1988;175(1):184–191. DOI: 10.1016/0014-4827(88)90265-0.
  10. Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, et al. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environmental and Molecular Mutagenesis. 2000;35(3):206–221. DOI: 10.1002/(SICI) 1098-2280(2000)35:3<206::AID-EM8>3.0.CO;2-J.
  11. Tyurin VA, Tyurina YY, Borisenko GG, Sokolova TV, Ritov VB, Quinn PJ, et al. Oxidative stress following traumatic brain injury in rats: quantitation of biomarkers and detection of free radical intermediates. Journal of Neurochemistry. 2000;75(5):2178–2189. DOI: 10.1046/j.1471-4159.2000.0752178.x.
  12. Ostling O, Johanson KJ. Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochemical and Biophysical Research Communications. 1984;123:291–298. DOI: 10.1016/0006-291x(84)90411-x.
  13. Filippov EV. Using the «DNA comet» method to detect and assess the degree of DNA damage in the cells of plants, animals and humans caused by environmental factors. Nauka i obrazovanie. 2014;2:72–78. Russian. EDN: SUFCTF.
  14. Zhanataev AK, Anisina EA, Chayka ZV, Miroshkina IA, Durnev AD. Phenomenon of atypical DNA comets. Tsitologiya. 2017; 59(3):163–168. Russian.
  15. Hartmann A, Speit G. The contribution of cytotoxicity to DNA-effects in the single cell gel test (comet assay). Toxicology Letters. 1997;90:183–188. DOI: 10.1016/S0378-4274(96)03847-7.
Published
2024-07-08
Keywords: HaCaT keratinocytes, p-benzoquinones, quercetin, microstructures, oxidative stress, DNA damage, DNA comets
How to Cite
Potapovich, A. I., Kostyuk, T. V., Shutava, T. G., & Kostyuk, V. A. (2024). Effect of quercetin and its microstructured forms on cell viability and nuclear DNA degradation in human keratinocytes when modelling oxidative stress. Experimental Biology and Biotechnology, 2, 46-54. Retrieved from https://journals.bsu.by/index.php/biology/article/view/6355
Issue
No 2 (2024): Experimental Biology and Biotechnology
Section
Physiology and Сell Biology

Copyright (c) 2024 Experimental Biology and Biotechnology

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

The authors who are published in this journal agree to the following:

  1. The authors retain copyright on the work and provide the journal with the right of first publication of the work on condition of license Creative Commons Attribution-NonCommercial. 4.0 International (CC BY-NC 4.0).
  2. The authors retain the right to enter into certain contractual agreements relating to the non-exclusive distribution of the published version of the work (e.g. post it on the institutional repository, publication in the book), with the reference to its original publication in this journal.
  3. The authors have the right to post their work on the Internet (e.g. on the institutional store or personal website) prior to and during the review process, conducted by the journal, as this may lead to a productive discussion and a large number of references to this work. (See The Effect of Open Access.)