Induction of resistance to salinization in rape plants under the influence of elicitors derived from Pseudomonas and Bacillus bacteria

Authors

  • Irina A. Grineva Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus
  • Yulia M. Kuleshova Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus
  • Veronica A. Lomonosova Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus
  • Diana V. Maslak Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus
  • Ludmila E. Sadovskaya Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus
  • Tatyana L. Skakun Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus
  • Irina N. Feklistova Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus
  • Natalia P. Maksimova Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus

Keywords:

Pseudomonas, Bacillus, elicitors, rape plants, salinization

Abstract

It was established that bacterial elicitors from P. chlororaphis subsp. aurantiaca 162, P. putida F19 and B. subtilis 494 are capable to increase both the seed vigor and germination of rape plants under high level of salinity condition (250 mmol/l NaCl). Сo-cultivation conditions of selected bacterial strains were optimized. The most effective elicitor’s variant capable to enhance the ability of the rape plants to generate induced systemic resistance (ISR) under salinity 150 mmol/l NaCl was selected. ISR manifested in a significant increasing of rape plants germination by 20 % and the stem length increased by 19 %. This test elicitor’s sample was prepared with heat treatment (100 °C, 15 min) of the culture broth containing P. chlororaphis subsp. aurantiaca 162 and B. subtilis 494 grown in M9 medium with molasses at 28 °C for 4 h and then at 35 °C to 48 h.

Author Biographies

  • Irina A. Grineva, Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus

    senior researcher at the sector of molecular genetics and biotechnology of microorganisms at the laboratory of molecular genetics and biotechnology at the department of genetics, faculty of biology.

  • Yulia M. Kuleshova, Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus

    PhD (biology); senior researcher at the sector of molecular genetics and biotechnology of microorganisms at the laboratory of molecular genetics and biotechnology at the department of genetics, faculty of biology.

  • Veronica A. Lomonosova, Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus

    researcher at the sector of molecular genetics and biotechnology of microorganisms at the laboratory of molecular genetics and biotechnology at the department of genetics, faculty of biology.

  • Diana V. Maslak, Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus

    head of the sector of molecular genetics and biotechnology of microorganisms at the laboratory of molecular genetics and biotechnology at the department of genetics, faculty of biology.

  • Ludmila E. Sadovskaya, Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus

    senior researcher at the sector of molecular genetics and biotechnology of microorganisms at the laboratory of molecular genetics and biotechnology at the department of genetics, faculty of biology.

  • Tatyana L. Skakun, Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus

    senior researcher at the sector of molecular genetics and biotechnology of microorganisms at the laboratory of molecular genetics and biotechnology at the department of genetics, faculty of biology. 

  • Irina N. Feklistova, Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus

    PhD (biology); head of the research laboratory of molecular genetics and biotechnology at the department of genetics, faculty of biology.

  • Natalia P. Maksimova, Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus

    doctor of science (biology), full professor; head of the department of genetics, faculty of biology.

References

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Published

2017-12-03

Issue

No. 1 (2017): Journal of the Belarusian State University. Biology

Section

Physiology and Сell Biology

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How to Cite

Grineva, I. A., Kuleshova, Y. M., Lomonosova, V. A., Maslak, D. V., Sadovskaya, L. E., Skakun, T. L., Feklistova, I. N., & Maksimova, N. P. (2017). Induction of resistance to salinization in rape plants under the influence of elicitors derived from Pseudomonas and Bacillus bacteria. Experimental Biology and Biotechnology, 1, 38-43. https://journals.bsu.by/index.php/biology/article/view/2426