The knockdown of the fusion oncogene KMT2A-AFF1 is associated with differential RNA splicing in human acute lymphoblastic leukemia cells

Authors

  • Vasily V. Grinev Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus
  • Olaf Heidenreich Wolfson Childhood Cancer Research Centre, Nothern Institute for Cancer Research, Newcastle University, Herschel Building, NE1 7RU, Newcastle upon Tyne, United Kingdom

Keywords:

fusion oncogene KMT2A-AFF1, knockdown of expression, alternative RNA splicing, human leukemia cells

Abstract

The fusion oncogene KMT2A-AFF1 is the outcome of translocation t (4; 11)(q21; q23). If this occurred at the level of early hematopoietic progenitors, this oncogene plays the role of one of the factors in the genesis of children acute lymphoblastic leukemia, the mechanism of which is not fully understood. In this work, it was shown that the KMT2A-AFF1 activity affects the state of transcriptome of leukemia cells. This effect is realized in two ways: through the differential expression of the target genes controlled by oncogene, and also through control of differential splicing. The presented results indicate the discovery of a new biological activity of oncogene KMT2A-AFF1, and it also opens new perspectives in study of t (4; 11)(q21; q23) positive leukemia.

Author Biographies

  • Vasily V. Grinev, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

    PhD (biology); associate professor at the department of genetics, faculty of biology

  • Olaf Heidenreich, Wolfson Childhood Cancer Research Centre, Nothern Institute for Cancer Research, Newcastle University, Herschel Building, NE1 7RU, Newcastle upon Tyne, United Kingdom

    PhD (philosophy); professor of molecular haematology

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Published

2018-05-01

Issue

No. 3 (2017): Journal of the Belarusian State University. Biology

Section

Genetics and Molecular Biology

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How to Cite

Grinev, V. V., & Heidenreich, O. . (2018). The knockdown of the fusion oncogene KMT2A-AFF1 is associated with differential RNA splicing in human acute lymphoblastic leukemia cells. Experimental Biology and Biotechnology, 3, 21-27. https://journals.bsu.by/index.php/biology/article/view/2457