Biochemical and molecular genetic characteristics of Bacillus pumilus bacteria isolated in Belarus

Authors

  • Olesia V. Evdokimova Institute of Microbiology, National Academy of Sciences of Belarus, 2 Kuprevič Street, Minsk 220141, Belarus
  • Vlad E. Miamin Institute of Microbiology, National Academy of Sciences of Belarus, 2 Kuprevič Street, Minsk 220141, Belarus; Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Leonid N. Valentovich Institute of Microbiology, National Academy of Sciences of Belarus, 2 Kuprevič Street, Minsk 220141, Belarus; Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

Keywords:

Bacillus pumilus, RAPD-PCR, REP-PCR, genetic heterogeneity
Supporting Agencies
The authors would like to thank E. I. Komar (Belarusian State University, department of the micro biology) and Y. N. Gorovik (department of the molecular biology) for provided B. pumilus strains.

Abstract

Physiological biochemical and molecular genetic properties of Bacillus pumilus bacteria isolated from affected plants and soil in Belarus have been studied. There were no significant phenotypical differences between bacteria isolated from plants and soil, only distinctions in cellulolytic and pectolytic activity were observed. However, RAPD- and REP-PCR typing revealed high genetic heterogeneity of studied isolates, which may indicate variability of genome structure of B. pumilus bacteria from various habitats.

Author Biographies

  • Olesia V. Evdokimova, Institute of Microbiology, National Academy of Sciences of Belarus, 2 Kuprevič Street, Minsk 220141, Belarus

    researcher at the laboratory «Center of analytical and genetic engineering research»

  • Vlad E. Miamin, Institute of Microbiology, National Academy of Sciences of Belarus, 2 Kuprevič Street, Minsk 220141, Belarus; Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    PhD (biology), docent; senior researcher at the laboratory of biological control agents, Institute of Microbiology, National Academy of Sciences of Belarus; associate professor at the department of microbiology, faculty of biology, Belarusian State University

  • Leonid N. Valentovich, Institute of Microbiology, National Academy of Sciences of Belarus, 2 Kuprevič Street, Minsk 220141, Belarus; Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    PhD (biology), docent; head of the laboratory «Center of analytical and genetic engineering research», Institute of Microbiology, National Academy of Sciences of Belarus; associate professor at the department of molecular biology, faculty of biology, Belarusian State University

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Published

2018-05-03

Issue

No. 1 (2018): Journal of the Belarusian State University. Biology

Section

Genetics, Microbiology and Molecular Biology

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How to Cite

Biochemical and molecular genetic characteristics of Bacillus pumilus bacteria isolated in Belarus. (2018). Experimental Biology and Biotechnology, 1, 38-49. https://journals.bsu.by/index.php/biology/article/view/2485