Regulation of phenolic secondary metabolites production by Echinacea purpurea L. Moench callus culture of root origin

Authors

  • Tatyana I. Ditchenko Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Vladimir M. Yurin Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

Keywords:

Echinacea purpurea L. Moench, callus of a root origin, growth index, phenolic compounds, hydroxycinnamic acids, flavonoids

Abstract

The protocol for obtaining a callus culture of purple coneflower (Echinacea purpurea L. Moench) of root origin from aseptically grown seedlings as a source of explants has been developed. To optimize the growth of callus culture biomass 10 combinations of synthetic auxins (2,4-D, NAA) and cytokinins (kinetin, BAP) have been tested. The levels of hydroxycinnamic acid and flavonoid accumulation in callus, cultivated in the presence of different combinations of phytohormones, are determined. It has been established that modification of the mineral base of the Murashige – Skoog (MS) nutrient medium (reduction of nitrate and phosphate concentration, complete exclusion of ammonium) makes it possible to significantly increase the content of a target secondary metabolites. It was shown that there is no stimulating effect of sucrose on the levels of phenylpropanoid and flavonoid accumulation.

Author Biographies

  • Tatyana I. Ditchenko, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    PhD (biology), docent; associate professor at the department of plant cell biology and bioengineering,
    faculty of biology

  • Vladimir M. Yurin, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    doctor of science (biology), full professor; professor at the department of plant cell biology and bioengineering, faculty of biology

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Published

2019-01-18

Issue

No. 2 (2018): Journal of the Belarusian State University. Biology

Section

Plant Cell Biology and Biotechnology

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How to Cite

Ditchenko, T. I., & Yurin, V. M. (2019). Regulation of phenolic secondary metabolites production by Echinacea purpurea L. Moench callus culture of root origin. Experimental Biology and Biotechnology, 2, 57-64. https://journals.bsu.by/index.php/biology/article/view/2509