Brassinolideand castasterone-induced modifications of growth processes and biomass accumulation in in vitro culture of Phalaenopsis × hybridum Blume protocorms

Authors

  • Maryia A. Charnysh Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Darya A. Przhevalskaya Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus b
  • Ilya A. Horski Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Dmitrii V. Kolbanov Teaching and Research Centre «Schemislitsa», Belarusian State University, 15-А Žukoŭskaha Street, Ščamyslica 223049, Minsk region, Belarus
  • Vladimir N. Zhabinskii Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, 5 Academician V. F. Kupreviča Street, Minsk 220141, Belarus
  • Vladimir A. Khripach Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, 5 Academician V. F. Kupreviča Street, Minsk 220141, Belarus
  • Vadim V. Demidchik Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

Keywords:

Phalaenopsis × hybridum Blume, brassinosteroids, seeds propagation, protocorms, in vitro culture

Abstract

Brassinosteroids is a class of plant steroid hormones, which mainly targets growth, development and stress responses in higher plants. Nevertheless, their effects on the growth of orchid plants and orchid in vitro cultures, which are used in biotechnology, have not been studied in details. The objective of this work was to characterize effects of major brassinosteroids, such as brassinolide and castasterone, on the growth and development of Phalaenopsis × hybridum Blume protocorms in vitro. The obtained data show that both brassinolide and castasterone cause significant increase of the physical size and weight of Phalaenopsis protocorms. As low as 0.1 nmol ⋅ L–1 of brassinolide and castasterone in the medium induced statistically significant stimulation of growth processes. 2–3-fold increase in biomass and length was induced
by addition of 0.1–1.0 µmol ⋅ L–1 of brassinolide and castasterone. Treatment by brassinosteroids increased the length of roots but did not change their quantity and developmental characteristics. Thus, results of this study clearly show that brassinosteroids are promising agents for regulation of orchid growth, which can act in extremely low concentrations and cause the pronounced stimulatory effects on growth but not on the development. Treatment by brassinosteroids as well as studying their effects on the physiological characteristics of ornamental orchids in vitro culture can potentially have an impact on the fundamental knowledge in the field of plant physiology and on the development of new biotechnologies.

Author Biographies

  • Maryia A. Charnysh, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    master ’s degree student at the department of plant cell biology and bioengineering, faculty of biology

  • Darya A. Przhevalskaya, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus b

    junior researcher at the research laboratory of plant cell biology and bioengineering, faculty of biology

  • Ilya A. Horski, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    student at the faculty of biology

  • Dmitrii V. Kolbanov, Teaching and Research Centre «Schemislitsa», Belarusian State University, 15-А Žukoŭskaha Street, Ščamyslica 223049, Minsk region, Belarus

    deputy director

  • Vladimir N. Zhabinskii, Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, 5 Academician V. F. Kupreviča Street, Minsk 220141, Belarus

    doctor of science (chemistry), docent; chief researcher at the laboratory of steroid chemistry

  • Vladimir A. Khripach, Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, 5 Academician V. F. Kupreviča Street, Minsk 220141, Belarus

    doctor of science (chemistry), full professor, academician of the National Academy of Sciences of Belarus; head of the laboratory of steroid chemistry

  • Vadim V. Demidchik, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    doctor of science (biology), docent; head of the department of plant cell biology and bioengineering, faculty of biology

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Published

2019-01-18

Issue

No. 2 (2018): Journal of the Belarusian State University. Biology

Section

Plant Cell Biology and Biotechnology

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How to Cite

Charnysh, M. A., Przhevalskaya, D. A., Horski, I. A., Kolbanov, D. V., Zhabinskii, V. N., Khripach, V. A., & Demidchik, V. V. (2019). Brassinolideand castasterone-induced modifications of growth processes and biomass accumulation in in vitro culture of Phalaenopsis × hybridum Blume protocorms. Experimental Biology and Biotechnology, 2, 105-113. https://journals.bsu.by/index.php/biology/article/view/2514