Expression analysis of acdS-gene of Pseudomonas putida B-37 in transgenic plants Nicotiana tabacum

Authors

  • Alesia A. Melnikava Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Alena A. Khramtsova Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus
  • Katsiaryna S. Karaleva Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus
  • Daria A. Rutkevich Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus
  • Tatsiana A. Kukulianskaya Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

Keywords:

ACC-deaminase, Nicotiana tabacum, acdS-gene, Pseudomonas putida

Abstract

In current work was realized the transfer of recombinant plasmid pBI121acdS, carring P. putida B-37 bacterial acdSgene to the A. tumefaciens AGL0 cells. Transgenic plants of N. tabacum were created by agrobacterial transformation. Integration of P. putida B-37 bacterial acdS-gene to transgenic plants of N. tabacum was verified by PCR analysis, using specific primers to present gene. Presence of target acdS-gene in transgenic plants genome was proved by RT-PCR analysis. With help of  Real-time PCR was shown the difference between reference gene and target P. putida B-37 bacterial acdSgene expression. Expression of target gene exceeded reference gene in 1.27 times, those fact proved expression of acdS-gene in plants on high level. Expression of the heterologous gene in N. tabacum plants was also proved by biochemical method of ACC-deaminase specific activity define.

Author Biographies

  • Alesia A. Melnikava, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    junior researcher at the department of genetics, faculty of biology

  • Alena A. Khramtsova, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

    PhD (biology); associate professor at the department of genetics, faculty of biology

  • Katsiaryna S. Karaleva, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

    student at the faculty of biology

  • Daria A. Rutkevich, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

    student at the faculty of biology

  • Tatsiana A. Kukulianskaya, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

    PhD (biology); associate professor at the department of biochemistry, faculty of biology

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Published

2019-03-14

Issue

No. 1 (2019): Journal of the Belarusian State University. Biology

Section

Genetics and Molecular Biology

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How to Cite

Melnikava, A. A., Khramtsova, A. A., Karaleva, K. S., Rutkevich, D. A., & Kukulianskaya, T. A. (2019). Expression analysis of acdS-gene of Pseudomonas putida B-37 in transgenic plants Nicotiana tabacum. Experimental Biology and Biotechnology, 1, 45-53. https://doi.org/10.33581/2521-1722-2019-1-45-53