DNA repair activation and apoptosis suppression in keratinocytes as a possible mechanism of cytoprotective action of plant polyphenols under conditions of UV-irradiation

Authors

  • Vladimir A. Kostyuk Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Alla I. Potapovich Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Tatyana O. Suhan Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Ahmed Albuhaydar Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Tatyana V. Shman Belarusian Research Center for Pediatric Oncology, Hematology and Immunology, 43 Frunzenskaja Street, Baraŭliany 223053, Baraŭlianski siel´saviet, Minsk region, Belarus
  • Tatyana I. Ermilova Belarusian Research Center for Pediatric Oncology, Hematology and Immunology, 43 Frunzenskaja Street, Baraŭliany 223053, Baraŭlianski siel´saviet, Minsk region, Belarus
  • Chiara de Luca «MEDENA AG», 16 Industriestrasse, Affoltern-am-Albis 8910, Switzerland

Keywords:

ultraviolet radiation in the C range, UVR, plant polyphenolic compounds, inflammatory mediators, keratinocytes, histone H2AX

Abstract

The work investigated the responses of cultured human keratinocytes to ultraviolet radiation in the C range (UVC) with and without a number of plant polyphenolic compounds. The experimental data obtained in this work indicate a cytoprotective effect of the PPs added immediately after UVC exposure. The cytoprotective activity of plant polyphenolic compounds was reduced in the series: acacetin, silybin, baikalein, leontopodium acid, quercetin, cyanidine chloride, taxifolin, trans-ferulic acid. The effect of UVC irradiation with and without of acacetin on the process of phosphorylation of histones H2AX, the triggering mechanism for the repair of single-stranded DNA damage, was also investigated in keratinocytes. It has been established that, H2AX phosphorylation is activated in response to UVC radiation and acacetin has a significant effect on the kinetics of this process. It is concluded that the PPs can reduce the destructive effect of UVR on the skin cells, activating the process of repairing genetic damage.

Author Biographies

  • Vladimir A. Kostyuk, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    doctor of science (chemistry), docent; head of the research laboratory of physiology, department of human and animal physiology, faculty of biology

  • Alla I. Potapovich, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    PhD (biology); leading researcher at the research laboratory of physiology, department of human and animal physiology, faculty of biology

  • Tatyana O. Suhan, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    PhD (biology); senior researcher at the research laboratory of physiology, department of human and animal physiology, faculty of biology

  • Ahmed Albuhaydar, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    postgraduate student at the department of human and animal physiology, faculty of biology

  • Tatyana V. Shman, Belarusian Research Center for Pediatric Oncology, Hematology and Immunology, 43 Frunzenskaja Street, Baraŭliany 223053, Baraŭlianski siel´saviet, Minsk region, Belarus

    PhD (biology); head of the laboratory of immunological research

  • Tatyana I. Ermilova, Belarusian Research Center for Pediatric Oncology, Hematology and Immunology, 43 Frunzenskaja Street, Baraŭliany 223053, Baraŭlianski siel´saviet, Minsk region, Belarus

    junior researcher at the laboratory of immunological research

  • Chiara de Luca, «MEDENA AG», 16 Industriestrasse, Affoltern-am-Albis 8910, Switzerland

    PhD (medicine); мedical and regulatory affairs manager

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Published

2019-06-27

Issue

No. 2 (2019): Journal of the Belarusian State University. Biology

Section

Cell Biology and Physiology

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How to Cite

Kostyuk, V. A., Potapovich, A. I., Suhan, T. O., Albuhaydar, A. ., Shman, T. V., Ermilova, T. I., & de Luca, C. . (2019). DNA repair activation and apoptosis suppression in keratinocytes as a possible mechanism of cytoprotective action of plant polyphenols under conditions of UV-irradiation. Experimental Biology and Biotechnology, 2, 29-35. https://doi.org/10.33581/2521-1722-2019-2-29-35