The contribution of various mechanisms to mRNA diversity of human fusion oncogene RUNX1-RUNX1T1

Authors

  • Ilya M. Ilyushonak Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus http://orcid.org/0000-0002-5377-366X (unauthenticated)
  • Alexandr A. Migas bBelarusian Research Center for Pediatric Oncology, Hematology and Immunology, 43 Frunzenskaja Street, Baraŭliany 223053, Baraŭlianski siel´saviet, Minsk region, Belarus
  • Andrei Yu. Sukhareuski nstitute of Cell Biology, University of Edinburgh, Roger Land Building, Alexander Crum Brown Road, EH9 3FF, Edinburgh, United Kingdom
  • Aksana D. Schneider University Hospital Carl Gustav Carus, Dresden University of Technology, 9 Mommsenstrasse, Dresden 01069, Germany
  • Vasily V. Grinev Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

Keywords:

fusion oncogene RUNX1-RUNX1T1, alternative RNA splicing, high throughput sequencing, biological noise
Supporting Agencies
This work is a part of sub-programme «Union» of the State Program on Scientific Research «Convergence-2020» (grant 3.08.03 (No. 469/54)).

Abstract

In this work, we used a comprehensive set of the fusion oncogene RUNX1-RUNX1T1 alternative exons to analyze the patterns of its mRNA generation. We found that the waste majority of alternative exons are modified variants of canonical exons, and the transcripts, including such exons, have a very low expression level. The «hot regions», including exons 4a, 6, 8b, 9, 11 and 12, produces about 80 % of such variants. Also we described a new transcription start region of RUNX1-RUNX1T1 and provide the evidences of co-expression of the fusion RNAs with normal and shortened 3′-UTRs in leukemic cells.

Author Biographies

  • Ilya M. Ilyushonak, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    assistant at the department of genetics, faculty of biology

  • Alexandr A. Migas, bBelarusian Research Center for Pediatric Oncology, Hematology and Immunology, 43 Frunzenskaja Street, Baraŭliany 223053, Baraŭlianski siel´saviet, Minsk region, Belarus

    senior researcher at the laboratory of immunological research

  • Andrei Yu. Sukhareuski, nstitute of Cell Biology, University of Edinburgh, Roger Land Building, Alexander Crum Brown Road, EH9 3FF, Edinburgh, United Kingdom

    postgraduate student

  • Aksana D. Schneider, University Hospital Carl Gustav Carus, Dresden University of Technology, 9 Mommsenstrasse, Dresden 01069, Germany

    PhD (biology); researcher at the section of medical systems biology, medical faculty

  • Vasily V. Grinev, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    PhD (biology), docent; associate professor at the department of genetics, faculty of biology

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Published

2019-07-01

Issue

No. 2 (2019): Journal of the Belarusian State University. Biology

Section

Genetics and Molecular Biology

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How to Cite

Ilyushonak, I. M., Migas, A. A., Sukhareuski, A. Y., Schneider, A. D., & Grinev, V. V. (2019). The contribution of various mechanisms to mRNA diversity of human fusion oncogene RUNX1-RUNX1T1. Experimental Biology and Biotechnology, 2, 45-59. https://doi.org/10.33581/2521-1722-2019-2-45-59