The influence of bacteria acdS-gene of Pseudomonas putida B-37 on Nicotiana tabacum transgenic plants under abiotic stress conditions

Authors

  • Daria A. Rutkevich Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus https://orcid.org/0000-0002-0096-7209 (unauthenticated)
  • Katsiaryna S. Karaleva Institute of Genetics and Cytology, National Academy of Sciences of Belarus, 27 Akademičnaja Street, Minsk 220072, Belarus
  • Elena A. Khramtsova Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

Keywords:

ACC-deaminase, Nicotiana tabacum, acdS-gene, Pseudomonas putida, abiotic stress

Abstract

In the present work was carried out selection of transgenic plants of Nicotiana tabacum carrying acdS-gene of bacteria Pseudomonas putida B-37. Using polymerase chain reaction with specific primers, the presence of the target gene was proved. Transgenic plants selected on a selective medium were planted in the ground and subjected to abiotic stress caused by soil contamination with heavy metal salts (15 mg / kg for Cu2+ and 30 mg / kg for Cr6+ and Pb2+ ) and salinization of the soil (200 mmol/L NaCl). Reverse transcription polymerase chain reaction and real-time polymerase chain reaction were conducted and confirmed the transcriptional activity of the bacterial acdS-gene in transgenic plant cells at a level with the reference gene Ef-1a. Determination of activity of ACC-deaminase, the product of expression of the acdS-gene, confirmed the formation of the active enzyme in the leaf tissues of transgenic tobacco plants. The beneficial effect of the acdS-gene of the bacteria P. putida B-37 on transgenic N. tabacum plants under abiotic stress has been proven.

Author Biographies

  • Daria A. Rutkevich, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    master’s degree student at the department of genetics, faculty of biology

  • Katsiaryna S. Karaleva, Institute of Genetics and Cytology, National Academy of Sciences of Belarus, 27 Akademičnaja Street, Minsk 220072, Belarus

    junior researcher

  • Elena A. Khramtsova, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    PhD (biology), docent; associate professor at the department of genetics, faculty of biology

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Published

2020-03-06

Issue

No. 1 (2020): Journal of the Belarusian State University. Biology

Section

Genetics and Molecular Biology

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How to Cite

Rutkevich, D. A. ., Karaleva, K. S. ., & Khramtsova, E. A. . (2020). The influence of bacteria acdS-gene of Pseudomonas putida B-37 on Nicotiana tabacum transgenic plants under abiotic stress conditions. Experimental Biology and Biotechnology, 1, 39-46. https://doi.org/10.33581/2521-1722-2020-1-39-46