The ability of plant polyphenols to reduce the number of UVC-induced single-stranded nuclear DNA breaks in keratinocytes

Authors

  • Alla I. Potapovich Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Ahmed Albuhaydar Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Sergey V. Gloushen Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Vladimir A. Kostyuk Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

Keywords:

UVC, PPs, single-stranded DNA breaks, DNA comets, HaCaT keratinocytes

Abstract

The effect of plant polyphenolic compounds (PPs) on the viability of human keratinocytes exposed to ultraviolet (UV) radiation of range C (UVС) and the number of single-stranded DNA breaks in the nuclei of these cells was studied. The experimental data obtained indicate that, along with the cytoprotective effect, the addition of silybin and acacetin immediately after the UVC exposure leads to a significant decrease in the number of single-stranded DNA breaks in the nuclei of HaCaT keratinocytes 2 and 5 h after exposure. It was concluded that PPs are able to reduce the destructive effect of UV radiation on skin cells, reducing the number of genetic damage.

Author Biographies

  • Alla I. Potapovich, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    PhD (biology); leading researcher at the research laboratory of physiology at the department of human and animal physiology, faculty of biology

  • Ahmed Albuhaydar, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    postgraduate student at the department of human and animal physiology, faculty of biology

  • Sergey V. Gloushen, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    PhD (biology), docent; associate professor at the department of genetics, faculty of biology

  • Vladimir A. Kostyuk, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    doctor of science (chemistry), docent; head of the research laboratory of physiology at the department of human and animal physiology, faculty of biology

References

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  5. Potapovic AI, Suhan TO, Albuhaydar А, Shman TV, Ermilova TI, de Luca C, Kostyuk VA. DNA repair activation and apoptosis suppression in keratinocytes as a possible mechanism of cytoprotective action of plant polyphenols under conditions of UV-irradiation. Journal of the Belarusian State University. Biology. 2019;2:29–35. Russian. DOI: 10.33581/2521-1722-2019-2-29-35.

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Published

2020-09-12

Issue

No. 2 (2020): Journal of the Belarusian State University. Biology

Section

Short Communications

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How to Cite

Potapovich, A. I. ., Albuhaydar, A. ., Gloushen, S. V. ., & Kostyuk, V. A. . (2020). The ability of plant polyphenols to reduce the number of UVC-induced single-stranded nuclear DNA breaks in keratinocytes. Experimental Biology and Biotechnology, 2, 84-89. https://doi.org/10.33581/2521-1722-2020-2-84-89