Sclerotinia sclerotiorum KK-1 isolate from Belarus: pathogenicity factors and glyphosate sensitivity

Authors

  • Alena V. Kulik Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus https://orcid.org/0000-0003-4651-7497
  • Margarita A. Shukshina Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Anatoliy N. Evtushenkov Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

Keywords:

Sclerotinia sclerotiorum, sclerotia formation, polygalacturonase activity, a-amylase activity, cellulase activity, glyphosate

Abstract

In the presence work, mycelial growth, sclerotia formation, polygalacturonase, a-amylase and cellulase activities of a Belarusian fungal isolate Sclerotinia sclerotiorum KK-1 collected from infected сarrot (Daucus carota subsp. sativus) were analyzed. It was established that polygalacturonase was induced by pectin and subject to catabolite repression by glucose. The ability of the isolate to produce oxalic acid, an important factor of pathogenesis, was observed throughout fungus incubation on PDA medium amended with bromophenol blue. Glyphosate (commercial counterpart «Tornado») starting from its level in the medium of 200 mg/L caused a significant inhibition of mycelial growth and sclerotia formation. It was observed that a-amylase and cellulase activities were inhibited by glyphosate unlike polygalacturonase activity.

Author Biographies

  • Alena V. Kulik, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    PhD (biology); head of the laboratory of transgenic plants, department of molecular biology, faculty of biology

  • Margarita A. Shukshina, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    student at the faculty of biology

  • Anatoliy N. Evtushenkov, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    doctor of science (biology), full professor; head of the department of molecular biology, faculty of biology

References

  1. Boland GJ, Hall R. Index of plant hosts of Sclerotinia sclerotiorum. Canadian Journal of Plant Pathology. 1994;16(2):93–108. DOI: 10.1080/07060669409500766.
  2. Bell JN, Ryder TB, Wingate VP, Bailey JA, Lamb CJ. Differential accumulation of plant defense gene transcripts in a comparable and an incomparable plant-pathogen interaction. Molecular and Cellular Biology. 1986;6(5):1615–1623. DOI: 10.1128/mcb.6.5.1615.
  3. Liang Y, Strelkov SE, Kav NNV. The proteome of liquid sclerotial exudates from Sclerotinia sclerotiorum. Journal of Proteome Research. 2010;9(6):3290–3298. DOI: 10.1021/pr900942w.
  4. Godoy G, Steadman JR, Dickman MB, Dam R. Use of mutants to demonstrate the role of oxalic acid in pathogenicity of Sclerotinia sclerotiorum on Phaseolus vulgaris. Physiological and Molecular Plant Pathology. 1990;37(3):179–191. DOI: 10.1016/0885- 5765(90)90010-U.
  5. Li R, Rimmer R, Buchwaldt L, Sharpe AG, Seguin-Swartz G, Hegedus DD. Interaction of Sclerotinia sclerotiorum with Brassica napus: сloning and characterization of endo- and exo-polygalacturonases expressed during saprophytic and parasitic modes. Fungal Genetics and Biology. 2004;41(8):754–765. DOI: 10.1016/j.fgb.2004.03.002.
  6. Bashi ZD, Rimmer SR, Khachatourians GG, Hegedus DD. Brassica napus polygalacturonase inhibitor proteins inhibit Sclerotinia sclerotiorum polygalacturonase enzymatic and necrotizing activities and delay symptoms in transgenic plants. Canadian Journal of Microbiology. 2013;59(2):79–86. DOI: 10.1139/cjm-2012-0352.
  7. Asoufi H, Hameed KM, Mahasneh A. The cellulase and pectinase activities associated with the virulence of indigenous Sclerotinia sclerotiorum isolates in Jordan Valley. The Plant Pathology Journal. 2007;23(4):233–238. DOI: 10.5423/PPJ.2007.23.4.233.
  8. Yajima W, Kav NN. The proteome of the phytopathogenic fungus Sclerotinia sclerotiorum. Proteomics. 2006;6(22):5995–6007. DOI: 10.1002/pmic.200600424.
  9. Oliveira MB, Barbosa SC, Petrofeza S. Comparative in vitro and in planta analyses of extracellular enzymes secreted by the pathogenic fungus Sclerotinia sclerotiorum. Genetics and Molecular Research. 2013;12(2):1796–1807. DOI: 10.4238/2013.June.6.3.
  10. Ellouze O, Mejri M, Smaali I, Limam F, Marzouki MN. Induction, properties and application of xylanase activity from Sclerotinia sclerotiorum S2 fungus. Journal of Food Biochemistry. 2007;31(1):96–107. DOI: 10.1111/j.1745-4514.2007.00101.x.
  11. Abdelmalek IB, Urdaci MC, Ali MB, Denayrolles M, Chaignepain S, Limam F, et al. Structural investigation and homology modeling studies of native and truncated forms of alpha-amylases from Sclerotinia sclerotiorum. Journal of Microbiology and Biotechnology. 2009;19(11):1306–1318. DOI: 10.4014/jmb.0903.3013.
  12. Iulek J, Franco OL, Silva M, Slivinski CT, Bloch CJr, Rigden DJ, et al. Purification, biochemical characterisation and partial primary structure of a new alpha-amylase inhibitor from Secale cereal (rye). The International Journal of Biochemistry and Cell Biology. 2000;32(11–12):1195–1204. DOI: 10.1016/s1357-2725(00)00053-4.
  13. Kassaby FY, Hepworth G. Phytophthora cinnamomi: effects of herbicide sonradial growth, sporangial production, inoculum potential and root disease in Pinus radiate. Soil Biology and Biochemistry. 1987;19(4):437–441. DOI: 10.1016/0038-0717(87)90035-6.
  14. Harris D, Grossbard E. Effects of the herbicides gramoxone W and roundup on Septorianodorum. Transactions of the British Mycological Society. 1979;73(1):27–33. DOI: 10.1016/S0007-1536(79)80068-6.
  15. Anderson JA, Kolmer JA. Rust control in glyphosate tolerant wheat following the application of the herbicide glyphosate. Plant Disease. 2005;89(11):1136–1142. DOI: 10.1094/PD-89-1136.
  16. Feng PCC, Baley J, ClintonWP, Bunkers GJ, Alibhai MF, Paulitz TC, et al. Glyphosate inhibits rust diseases in glyphosate-resistant wheat and soybean. Proceedings of the National Academy of Sciences of the United States of America. 2005;102(48):17290–17295. DOI: 10.1073/pnas.0508873102.
  17. Westerhuis D, Vawdrey LL, Piper R. An in vitro study into the effect of glyphosate on Sclerotium rolfsii. Australasian Plant Disease Notes. 2007;2(1):23–24. DOI: 10.1071/DN07010.
  18. Attanayake RN, Carter PA, Jiang D, Del Río-Mendoza L, Chen W. Sclerotinia sclerotiorum populations infecting canola from China and the United States are genetically and phenotypically distinct. Phytopathology. 2013;103(7):750–761. DOI: 10.1094/PHYTO-07-12-0159-R.
  19. Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry. 1959;31(3):426–428. DOI: 10.1021/ac60147a030.
  20. Dawson R, Elliot D, Elliot U, Dzhons M. Spravochnik biokhimika [Biochemist’s handbook]. Drutsa VL, Koroleva VN, translators. Мoscow: Mir; 1991. 544 p. Russian.
  21. Naher N, Shamsi S, Rawshan Ali MD, Nahar MS, Bashar MA. Interrelation of oxalic acid formation with pathogenecity of Sclerotinia sclerotiorim (Lib.) de Bary causing white mold disease of mustard. Bangladesh Journal of Botany. 2017;46(3):963–970.
  22. Favaron F, Sella L, D’Ovidio R. Relationships among endo-polygalacturonase, oxalate, pH, and plant polygalacturonaseinhibiting protein (PGIP) in the interaction between Sclerotinia sclerotiorum and soybean. Molecular Plant-Microbe Interactions. 2004;17(12):1402–1409. DOI: 10.1094/mpmi.2004.17.12.1402.
  23. Fraissinet-Tachet L, Fevre M. Regulation by galacturonic acid of pectinolytic enzyme production by Sclerotinia sclerotiorum. Current Microbiology. 1996;33(1):49–53. DOI: 10.1007/s002849900073.
  24. Riou C, Fraissinet-Tachet L, Freyssinet G, Fèvre M. Secretion of pectic isoenzymes by Sclerotinia sclerotiorum. FEMS Microbiology Letters. 1992;91(3):231–237. DOI: 10.1111/J.1574-6968.1992.TB05214.X.

Downloads

Additional Files

Published

2020-12-31

Issue

No. 3 (2020): Journal of the Belarusian State University. Biology

Section

Cell Biology and Physiology

License

The authors who are published in this journal agree to the following:

  1. The authors retain copyright on the work and provide the journal with the right of first publication of the work on condition of license Creative Commons Attribution-NonCommercial. 4.0 International (CC BY-NC 4.0).
  2. The authors retain the right to enter into certain contractual agreements relating to the non-exclusive distribution of the published version of the work (e.g. post it on the institutional repository, publication in the book), with the reference to its original publication in this journal.
  3. The authors have the right to post their work on the Internet (e.g. on the institutional store or personal website) prior to and during the review process, conducted by the journal, as this may lead to a productive discussion and a large number of references to this work. (See The Effect of Open Access.)

How to Cite

Kulik, A. V., Shukshina, M. A., & Evtushenkov, A. N. (2020). Sclerotinia sclerotiorum KK-1 isolate from Belarus: pathogenicity factors and glyphosate sensitivity. Experimental Biology and Biotechnology, 3, 54-63. https://doi.org/10.33581/2521-1722-2020-3-54-69