Effects of 2-amino-4,6-di-tert-butylphenol derivatives on the viability and functional state of human peripheral blood lymphocytes

Authors

  • Darya B. Nizheharodava International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus https://orcid.org/0000-0003-1633-7487
  • Galina A. Ksendzova Research Institute of Physical and Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus
  • Aliaksei G. Sysa International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus
  • Mariya Yu. Yurkevich International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus
  • Maryna V. Labai International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus
  • Оleg I. Shadyro Research Institute of Physical and Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus
  • Marina M. Zafranskaya International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus

Keywords:

2-amino-4,6-di-tert-butylphenol derivatives, immune system, lymphoid cells, interferons, proliferation, cytotoxicity
Supporting Agencies
The authors are grateful to the Ministry of Education of the Republic of Belarus for financial supporting of research as part of the scientific work «Development of innovative targeted antiHIV-agents» (state registration No. 20191188).

Abstract

Derivatives of 2-amino-4,6-di-tert-butylphenol exhibit antiviral properties and radical regulatory activity against various types of organic radicals which determines the actuality of their further investigation. But the question of aminophenol derivatives immunomodulatory activity remains open. In this regard, the aim of the study was to assess the effects of 2-amino-4,6-di-tert-butylphenol derivatives on the viability and functional potential of human peripheral blood lymphocytes. As a result of the studies, it was shown that aminophenol compounds at concentrations of 10–5–10–7 mol did not exert a toxic effect while at a concentration of 10–4 mol showed a cytotoxic effect due to the induction of secondary necrosis. Compounds N-(2-hydroxy-3,5-di-tert-butylphenyl)-4-methylbenzenesulfonamide and 2,4-di-tert-butyl-6-morpholinophenol at a concentration of 10–6 mol stimulated the extracellular production of α-interferon by peripheral blood mononuclear cells and intracellular production of γ-interferon by CD3+T-lymphocytes. An immunosuppressive effect (more than 50 %) of N-(2-hydroxy-3,5-di-tert-butylphenyl)-4-methylbenzenesulfonamide and 2,4-di-tert-butyl-6-morpholinophenol compounds at a concentration of 10–5 mol was revealed to the mitogen-induced proliferation of T-lymphocytes.

Author Biographies

  • Darya B. Nizheharodava, International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus

    PhD (biology), docent; associate professor at the department of immunology, faculty of environmental medicine

  • Galina A. Ksendzova, Research Institute of Physical and Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus

    PhD (chemistry); leading researcher at the free radical processes chemistry laboratory

  • Aliaksei G. Sysa, International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus

    PhD (chemistry), docent; dean of the faculty of environmental medicine

  • Mariya Yu. Yurkevich, International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus

    PhD (biology); associate professor at the department of immunology, faculty of environmental medicine

  • Maryna V. Labai, International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus

    lecturer at the department of immunology, faculty of environmental medicine

  • Оleg I. Shadyro, Research Institute of Physical and Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus

    doctor of science (chemistry), full professor; head of the free radical processes chemistry laboratory

  • Marina M. Zafranskaya, International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus

    doctor of science (medicine), docent; head of the department of immunology, faculty of environmental medicine

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Published

2020-12-31

Issue

No. 3 (2020): Journal of the Belarusian State University. Biology

Section

Cell Biology and Physiology

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How to Cite

Nizheharodava, D. B., Ksendzova, G. A., Sysa, A. G., Yurkevich, M. Y., Labai, M. V., Shadyro О. I., & Zafranskaya, M. M. (2020). Effects of 2-amino-4,6-di-tert-butylphenol derivatives on the viability and functional state of human peripheral blood lymphocytes. Experimental Biology and Biotechnology, 3, 19-28. https://doi.org/10.33581/2521-1722-2020-3-19-28