Biocompatibility of a decellularized liver scaffold in studies in vitro

Authors

  • Mariya Yu. Yurkevich International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus https://orcid.org/0000-0002-5082-5874
  • Andrei D. Dubko International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus
  • Darya B. Nizheharodava International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus
  • Maryna V. Labai International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus
  • Marina M. Zafranskaya International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus

Keywords:

decellularization, perfusion-detergent method, liver scaffold, biocompatibility, multipotent mesenchymal stromal cells, splenocytes
Supporting Agencies
This work was supported by the Belarusian Republican Foundation for Fundamental Research (grant No. M19ARM-016 dated 02.05.2019).

Abstract

The development of multicomponent threedimensional structures based on decellularized tissue is a perspective alternative for organ transplantation in end-stage liver disease. The technology of rat liver decellularization is presented which consist in sequential perfusion of organ through the portal vein and use of 0.1 % sodium dodecyl sulfate as an active solution. The absence of the cytotoxic effect of decellularized scaffolds on allogeneic splenocytes and multipotent mesenchymal stromal cells was established. The obtained liver scaffolds are biocompatible in cells cultures and correspond criteria for cell carriers.

Author Biographies

  • Mariya Yu. Yurkevich, International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus

    PhD (biology); associate professor at the department of immunology, faculty of environmental medicine

  • Andrei D. Dubko, International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus

    junior researcher at the research sector

  • Darya B. Nizheharodava, International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus

    PhD (biology), docent; associate professor at the department of immunology, faculty of environmental medicine

  • Maryna V. Labai, International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus

    senior lecturer at the department of immunology, faculty of environmental medicine

  • Marina M. Zafranskaya, International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus

    doctor of science (medicine), docent; head of the department of immunology, faculty of environmental medicine

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Published

2021-03-12

Issue

No. 1 (2021): Journal of the Belarusian State University. Biology

Section

Cell Biology and Physiology

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How to Cite

Yurkevich, M. Y. ., Dubko, A. D. ., Nizheharodava, D. B. ., Labai, M. V. ., & Zafranskaya, M. M. . (2021). Biocompatibility of a decellularized liver scaffold in studies in vitro. Experimental Biology and Biotechnology, 1, 20-27. https://doi.org/10.33581/2521-1722-2021-1-20-27