Features of infrared spectra of blood plasma of patients with dyslipidemia

Authors

  • Elena V. Korolik Belarusian State Medical University, 83 Dziaržynski Avenue, Minsk 220116, Belarus
  • Marina V. Goltseva Belarusian State Medical University, 83 Dziaržynski Avenue, Minsk 220116, Belarus
  • Vera A. Zhukovskaya Belarusian State Medical University, 83 Dziaržynski Avenue, Minsk 220116, Belarus
  • Galina G. Lubnevskaya Belarusian State Medical University, 83 Dziaržynski Avenue, Minsk 220116, Belarus
  • Alexander A. Ivanov Belarusian State Medical University, 83 Dziaržynski Avenue, Minsk 220116, Belarus

Keywords:

IR spectroscopy, fluorescence sensing, dyslipidemia, albumin, low-density lipoproteins, very low-density lipoproteins

Abstract

The paper analyzes the IR spectra of blood plasma from healthy donors, patients with brain infarction and patients with coronary heart disease (CHD). It was found that the structure of the main transport protein of blood plasma – albumin – in the blood plasma of patients with brain infarction, patients with CHD and representatives of the control group is almost identical. It is shown that in the absence of changes in the secondary and tertiary structure of albumin, the increase in binding of anionic hydrophobic metabolites in the blood plasma of patients with dyslipidemia compared to control is due to structural changes in low-density lipoproteins (LDL) and very low-density lipoproteins (VLDL).

Author Biographies

  • Elena V. Korolik, Belarusian State Medical University, 83 Dziaržynski Avenue, Minsk 220116, Belarus

    PhD (physics and mathematics); associate professor at the department of medical and biological physics, pharmaceutical faculty

  • Marina V. Goltseva, Belarusian State Medical University, 83 Dziaržynski Avenue, Minsk 220116, Belarus

    senior lecturer at the department of medical and biological physics, pharmaceutical faculty

  • Vera A. Zhukovskaya, Belarusian State Medical University, 83 Dziaržynski Avenue, Minsk 220116, Belarus

    senior lecturer at the department of medical and biological physics, pharmaceutical faculty

  • Galina G. Lubnevskaya, Belarusian State Medical University, 83 Dziaržynski Avenue, Minsk 220116, Belarus

    senior lecturer at the department of medical and biological physics, pharmaceutical faculty

  • Alexander A. Ivanov, Belarusian State Medical University, 83 Dziaržynski Avenue, Minsk 220116, Belarus

    PhD (physics and mathematics); associate professor at the department of medical and biological physics, pharmaceutical faculty

References

  1. Shaw RA, Mantsch HH. Infrared spectroscopy in clinical and diagnostic analysis. In: Meyers RA, editor. Encyclopedia of analytical chemistry: applications, theory and instrumentation. Volume 1. Chichester: John Wiley and Sons; 2000. [p. 83–102].
  2. Ivanov AI, Korolenko EA, Korolik EV, Firsov SP, Zhbankov RG, Marchewka MK, et al. Chronic liver and renal diseases differently affect structure of human serum albumin. Archives of Biochemistry and Biophysics. 2002;408(1);69–77. DOI: 10.1016/S0003- 9861(02)00533-7.
  3. Krilov D, Balarin M, Kosović M, Gamulin O, Brnjas-Kraljević J. FT-IR spectroscopy of lipoproteins – a comparative study. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2009;73(4):701–706. DOI: 10.1016/j.saa.2009.03.015.
  4. Aronov DM. Lechenie i profilaktika ateroskleroza [Treatment and prevention of atherosclerosis]. Moscow: Triada-X; 2000. 411 p. Russian.
  5. Korolik AK, Dikevich AE, Oganova EG, Korolenko EA, Korolik EV, Ivanov AA. [Structural and functional state of the main transport proteins of the blood plasma of patients with dyslipidemia]. In: Kirkovskii VV, editor. Ekstrakorporal’naya gemokorrektsiya v intensivnoi terapii kriticheskikh sostoyanii. Tezisy dokladov 6-i Mezhdunarodnoi nauchno-prakticheskoi konferentsii; 23 maya 2013 g.; Minsk, Belarus [Extra-corporeal hemocorrection in intensive care of critical conditions. Collection of reports of the 6th International scientific and practical conference; 2013 May 23; Minsk, Belarusʼ]. Minsk: Belarusian State Medical University; 2013. p. 89–92. Russian.
  6. Korolenko EA, Korolik EV, Korolik AK, Kirkovskij VV. Estimation of the binding ability of main transport proteins of blood plasma at liver cirrhosis by the method of fluorescent probes. Journal of Applied Spectroscopy. 2007;74(4):507–511. Russian.
  7. Korolik EV, Korolenko EA, Tretinnikov ON, Kozlyakova OV, Korolik AK, Kirkovskiy VV. Dynamics of lipoproteins level in blood plasma of pregnant women against gestational age according to FTIR spectroscopy data. Journal of Applied Spectroscopy. 2012;79(6);933–937. Russian.
  8. Formazyuk VE, Dobretsov GE, Vladimirov YuA. [Change in the surface charge of lipoproteins isolated from the blood plasma of individuals with hyperalphalipoproteinemia]. Voprosy meditsinskoi khimii. 1981;27(1):125–128. Russian.

Downloads

Additional Files

Published

2021-03-12

Issue

No. 1 (2021): Journal of the Belarusian State University. Biology

Section

Short Communications

License

The authors who are published in this journal agree to the following:

  1. The authors retain copyright on the work and provide the journal with the right of first publication of the work on condition of license Creative Commons Attribution-NonCommercial. 4.0 International (CC BY-NC 4.0).
  2. The authors retain the right to enter into certain contractual agreements relating to the non-exclusive distribution of the published version of the work (e.g. post it on the institutional repository, publication in the book), with the reference to its original publication in this journal.
  3. The authors have the right to post their work on the Internet (e.g. on the institutional store or personal website) prior to and during the review process, conducted by the journal, as this may lead to a productive discussion and a large number of references to this work. (See The Effect of Open Access.)

How to Cite

Korolik, E. V. ., Goltseva, M. V. ., Zhukovskaya, V. A. ., Lubnevskaya, G. G. ., & Ivanov, A. A. . (2021). Features of infrared spectra of blood plasma of patients with dyslipidemia. Experimental Biology and Biotechnology, 1, 106-111. https://doi.org/10.33581/2521-1722-2021-1-106-111