Study of the ascorbate-glutathione cycle in transgenic plants Nicotiana tabacum under abiotic stress conditions

Authors

  • Kristina V. Pristupa Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus https://orcid.org/0000-0003-1977-4949
  • Tatsiana A. Kukulianskaya Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Elena A. Khramtsova Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

Keywords:

antioxidant system, ascorbate-glutathione cycle, acdS-gene, Nicotiana tabacum

Abstract

We conducted a comparative analysis of biochemical parameters for the ascorbate-glutathione cycle in non-transgenic and transgenic Nicotiana tabacum, plants cultivated in heavy metal polluted soils. Transgenic plants had in their genome a bacterial acdS-gene encoding the 1-aminocyclopropane-1-carboxylate deaminase (ACC-deaminase) enzyme. The introduction of elevated concentrations of copper, chromium, and lead ions into the soil promotes induction of the acdS-gene expression and an increase in ACC-deaminase activity in transgenic plants. It was shown the content of ascorbic acid (АА), glutathione, ascorbate peroxidase (АРХ) and glutathione peroxidase (GP) and glutathione reductase (GR) increased in plants under abiotic stress.

Author Biographies

  • Kristina V. Pristupa, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    postgraduate student at the department of biochemistry, faculty of biology

  • Tatsiana A. Kukulianskaya, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

     PhD (biology), docent; associate professor at the department of biochemistry, faculty of biology

  • Elena A. Khramtsova, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

     PhD (biology), docent; associate professor at the department of genetics, faculty of biology

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Published

2021-06-23

Issue

No. 2 (2021): Journal of the Belarusian State University. Biology

Section

Cell Biology and Physiology

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How to Cite

Pristupa, K. V., Kukulianskaya, T. A., & Khramtsova, E. A. (2021). Study of the ascorbate-glutathione cycle in transgenic plants Nicotiana tabacum under abiotic stress conditions. Experimental Biology and Biotechnology, 2, 11-18. https://doi.org/10.33581/2521-1722-2021-2-11-18