ATP synthase in rat brain neurons

Authors

Keywords:

ATP synathse, neurons, immunohistochemistry, brain, rat

Abstract

Mitochondrial ATP synthase is the main enzyme of ATP synthesis. A large number of diseases, including neurodegenerative ones, are associated with disorders of its assembly and functioning. The aim of the work is to reveal the patterns and features of the regional distribution of ATP synthase in the neurons of the rat brain structures, with a quantitative assessment of its content at the cellular level. The study was carried out on material from 5 outbred white male rats. Paraffin sections were stained by the Nissl method to identify structures of the brain and cervical segments of the spinal cord, and for ATP synthase by immunohistochemical method; 102 structures of the rat brain were studied cytophotometrically. Immunoreactivity of ATP synthase was found in all structures of the rat brain with high heterogeneity, while most structures are characterised with a moderate content of ATP synthase. A high content of ATP synthase was found in large neurons and in neurons of structures of phylogenetically older brain divisions. The content of ATP synthase also depends on the layer of the cortex and the position of neurons in the reflex arc. In the spinal cord the content of ATP synthase depends not only on the size of neurons, but also on the gray matter layer.

Author Biographies

  • Elizaveta V. Uzlova, Grodno State Medical University, 80 M. Horkaha Street, Hrodna 230009, Belarus

    postgraduate student at the department of histology, cytology and embryology, faculty of pediatrics

  • Siarhei M. Zimatkin, Grodno State Medical University, 80 M. Horkaha Street, Hrodna 230009, Belarus

    doctor of science (biology), full professor; head of the department of histology, cytology and embryology, faculty of pediatrics

References

  1. Capaldi RA, Aggeler R. Mechanism of the F1 Fo-type ATP synthase, a biological rotary motor. Trends in Biochemical Sciences. 2002;27(3):154–160. DOI: 10.1016/S0968-0004(01)02051-5.
  2. Paumard P, Vaillier J, Coulary B, Schaeffer J, Soubannier V, Mueller DM, et al. The ATP synthase is involved in generating mitochondrial cristae morphology. The EMBO Journal. 2002;21(3):221–230. DOI: 10.1093/emboj/21.3.221.
  3. Campanella M, Casswell E, Chong S, Farah Z, Wieckowski MR, Abramov AY, et al. Regulation of mitochondrial structure and function by the F1 Fo-ATPase inhibitor protein, IF1. Cell Metabolism. 2008;8(1):13–25. DOI: 10.1016/j.cmet.2008.06.001.
  4. García JJ, Ogilvie I, Robinson BH, Capaldi RA. Structure, functioning, and assembly of the ATP synthase in cells from patients with the T8993G mitochondrial DNA mutation. Comparison with the enzyme in Rho(0) cells completely lacking mtDNA. Journal of Biological Chemistry. 2000;275(15):11075–11081. DOI: 10.1074/jbc.275.15.11075.
  5. Reed T, Perluigi M, Sultana R, Pierce WM, Klein JB, Turner DM, et al. Redox proteomic identification of 4-hydroxy-2-nonenalmodified brain proteins in amnestic mild cognitive impairment: insight into the role of lipid peroxidation in the progression and pathogenesis of Alzheimer’s disease. Neurobiology of Disease. 2008;30(1):107–120. DOI: 10.1016/j.nbd.2007.12.007.
  6. Kucharczyk R, Zick M, Bietenhader M, Rak M, Couplan E, Blondel M, et al. Mitochondrial ATP synthase disorders: molecular mechanisms and the quest for curative therapeutic approaches. Biochimica et Biophysica Acta – Molecular Cell Research. 2009;1793(1):186–199. DOI: 10.1016/j.bbamcr.2008.06.012.
  7. Terni B, Boada J, Portero-Otin M, Pamplona R, Ferrer I. Mitochondrial ATP synthase in the entorhinal cortex is a target of oxidative stress as stages I/II of Alzheimer’s disease pathology. Brain Pathology. 2010;20(1):222–233. DOI: 10.1111/j.1750- 3639.2009.00266.x.
  8. Filosto M, Scarpelli M, Cotelli MS, Vielmi V, Todeschini A, Gregorelli V, et al. The role of mitochondria in neurodegenerative diseases. Journal of Neurology. 2011;258(10):1763–1774. DOI: 10.1007/s00415-011-6104-z.
  9. Federico A, Cardaioli E, Da Pozzo P, Formichi P, Gallus GN, Radi E. Mitochondria, oxidative stress and neurodegeneration. Journal of the Neurological Sciences. 2012;322(1–2):254–262. DOI: 10.1016/j.jns.2012.05.030.
  10. Dautant A, Meier T, Hahn A, Tribouillard-Tanvier D, di Rago J-P, Kucharczyk R. ATP synthase diseases of mitochondrial genetic origin. Frontiers in Physiology. 2018;9:329. DOI: 10.3389/fphys.2018.00329.
  11. Magistretti PJ, Pellerin L. Cellular mechanisms of brain energy metabolism. Relevance to functional brain imaging and to neurodegenerative disorders. Annals of the New York Academy of Sciences. 1996;777(1):380–387. DOI: 10.1111/j.1749-6632.1996. tb34449.x.
  12. Uzlova EV, Zimatkin SM. Distribution of ATP synthase in rat brain structures. Vestnik Smolenskoi gosudarstvennoi meditsinskoi akademii. 2020;19(3):11–16. Russian. DOI: 10.37903/vsgma.2020.3.2.
  13. Korzhevskii DE, Gilerovich EG, Kirik OV, Grigor’ev IP, Sukhorukova EG, Alekseeva OS, et al. Immunogistokhimicheskoe issledovanie golovnogo mozga [Immunohistochemical study of the brain]. Korzhevskii DE, editor. Saint Petersburg: SpetsLit; 2016. 143 p. Russian.
  14. Paxinos G, Watson C. The rat brain in stereotaxic coordinates. 6th edition. Amsterdam: Academic Press; 2007. [448 p.].
  15. Yamaguchi K, Ueda K. Rhythmic discharge of mitral cells in the carp olfactory bulb. Brain Research. 1984;322(2):378–381. DOI: 10.1016/0006-8993(84)90138-0.
  16. Bayer SA, Yackel JW, Puri PS. Neurons in the rat dentate gyrus granular layer substantially increase during juvenile and adult life. Science. 1982;216(4548):890–892. DOI: 10.1126/science.7079742.
  17. Mameli O, Caria MA, Biagi F, Zedda M, Farina V. Neurons within the trigeminal mesencephalic nucleus encode for the kinematic parameters of the whisker pad macrovibrissae. Physiological Reports. 2017;5(10):e13206. DOI: 10.14814/phy2.13206.
  18. Baker R, Llinás R. Electrotonic coupling between neurones in the rat mesencephalic nucleus. The Journal of Physiology. 1971; 212(1):45–63. DOI: 10.1113/jphysiol.1971.sp009309.
  19. Carricondo F, Romero-Gómez B. The cochlear spiral ganglion neurons: the auditory portion of the VIII nerve. The Anatomical Record. 2019;302(3):463–471. DOI: 10.1002/ar.23815.

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Published

2021-10-11

Issue

No. 3 (2021): Journal of the Belarusian State University. Biology

Section

Physiology and Сell Biology

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How to Cite

Uzlova, E. V., & Zimatkin, S. M. (2021). ATP synthase in rat brain neurons. Experimental Biology and Biotechnology, 3, 17-27. https://doi.org/10.33581/2521-1722-2021-3-17-27