Characteristics of cryoprotectors used for long-term storage of donor dendritic cells

Authors

  • Oksana V. Timohina Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, 27 Akademičnaja Street, Minsk 220072, Belarus https://orcid.org/0000-0001-7208-1637
  • Andrei Y. Hancharou Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, 27 Akademičnaja Street, Minsk 220072, Belarus https://orcid.org/0000-0002-4869-9864

Keywords:

dendritic cells, donor dendritic cells, cryoprotector

Abstract

Monocyte-derived donor dendritic cells are promising for use in the treatment of cancer. However, there are some problems that currently limit their clinical use. One of which is the cryopreservation of cells followed by restoration on demand. А cryoprotector must be added to the nutrient medium in order to reduce or completely eliminate the damaging factors acting on cells during freezing. Cryoprotectors refer to a wide range of sugars, diols and amino acids that stabilise biomolecules in various ways, depending on their molecular weight and mechanism of action on cells. The work describes groups of cryoprotectors (endo- and exocellular, mixed and combined cryoprotectors), as well as presents techniques of cryopreservation of dendritic cells.

Author Biographies

  • Oksana V. Timohina, Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, 27 Akademičnaja Street, Minsk 220072, Belarus

    junior researcher at the laboratory of immunology and cell biophysic

  • Andrei Y. Hancharou, Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, 27 Akademičnaja Street, Minsk 220072, Belarus

    PhD (medicine), docent; director

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Published

2021-10-22

Issue

No. 3 (2021): Journal of the Belarusian State University. Biology

Section

Short Communications

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How to Cite

Timohina, O. V., & Hancharou, A. Y. (2021). Characteristics of cryoprotectors used for long-term storage of donor dendritic cells. Experimental Biology and Biotechnology, 3, 102-108. https://doi.org/10.33581/2521-1722-2021-3-102-108