Development of a microsatellite panel for identification of biological samples of moose (Alces alces) in forensic research

Authors

  • Diana E. Nedzvedskaya Scientific and Practical Center of the State Forensic Examination Committee of the Republic of Belarus, 25 Filimonava Street, Minsk 220114, Belarus
  • Sviatlana A. Kotava Scientific and Practical Center of the State Forensic Examination Committee of the Republic of Belarus, 25 Filimonava Street, Minsk 220114, Belarus
  • Tatsiana V. Zabauskaya Scientific and Practical Center of the State Forensic Examination Committee of the Republic of Belarus, 25 Filimonava Street, Minsk 220114, Belarus
  • Veronika I. Rybakova Scientific and Practical Center of the State Forensic Examination Committee of the Republic of Belarus, 25 Filimonava Street, Minsk 220114, Belarus
  • Aliaksandra Ya. Hrebianchuk Scientific and Practical Center of the State Forensic Examination Committee of the Republic of Belarus, 25 Filimonava Street, Minsk 220114, Belarus
  • Iosif S. Tsybovsky BelJurZabespjachjenne, 2b Dziaržynskaha Avenue, Minsk 220069, Belarus

Keywords:

microsatellites, polymorphism, forensic identification, moosе

Abstract

For forensic analysis of evidence from illegal hunting sites of moose, a panel of 15 microsatellite (4 tetranucleotide and 11 dinucleotide) loci and 1 sex locus (amelogenin) was proposed. The cumulative power of discrimination of individual based on the common genetic database of moose (for a panel of 15 microsatellite loci) is more than 99.999 999999 999 %. The genetic and forensic indicators of a microsatellite panel were evaluated on a population sample of moose on a national scale (383 samples) for the first time in Europe and forensic methodology of the DNA identification of the moose (Alces alces) introduced into the national legal system.

Author Biographies

  • Diana E. Nedzvedskaya, Scientific and Practical Center of the State Forensic Examination Committee of the Republic of Belarus, 25 Filimonava Street, Minsk 220114, Belarus

    researcher at the laboratory of molecular biology research

  • Sviatlana A. Kotava, Scientific and Practical Center of the State Forensic Examination Committee of the Republic of Belarus, 25 Filimonava Street, Minsk 220114, Belarus

    PhD (chemistry); leading researcher at the laboratory of technical and criminalistic research, scientific department of technical, criminalistic and special research

  • Tatsiana V. Zabauskaya, Scientific and Practical Center of the State Forensic Examination Committee of the Republic of Belarus, 25 Filimonava Street, Minsk 220114, Belarus

    senior researcher at the laboratory of molecular biology research

  • Veronika I. Rybakova, Scientific and Practical Center of the State Forensic Examination Committee of the Republic of Belarus, 25 Filimonava Street, Minsk 220114, Belarus

    researcher at the laboratory of molecular biology research

  • Aliaksandra Ya. Hrebianchuk, Scientific and Practical Center of the State Forensic Examination Committee of the Republic of Belarus, 25 Filimonava Street, Minsk 220114, Belarus

    researcher at the laboratory of molecular biology research

  • Iosif S. Tsybovsky, BelJurZabespjachjenne, 2b Dziaržynskaha Avenue, Minsk 220069, Belarus

    leading specialist at the sector of educational and methodical work, division of human resources and educational and methodical work

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Published

2022-06-23

Issue

No. 2 (2022): Experimental Biology and Biotechnology

Section

Genetics and Molecular Biology

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How to Cite

Nedzvedskaya, D. E., Kotava, S. A., Zabauskaya, T. V., Rybakova, V. I., Hrebianchuk, A. Y., & Tsybovsky, I. S. (2022). Development of a microsatellite panel for identification of biological samples of moose (Alces alces) in forensic research. Experimental Biology and Biotechnology, 2, 27-36. https://doi.org/10.33581/2957-5060-2022-2-27-36