Phenotypic characteristics of bssS mutant of phytopathogenic bacteria Erwinia amylovora

Authors

  • Karina Yu. Pesotskaya Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus
  • Alexander L. Lagonenko Algimed Techno, 22/1 Lagojski Tract, Minsk 220090, Belarus
  • Anatoli N. Evtushenkov Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus

Keywords:

Erwinia amylovora, BssS regulator, cell motility, biofilm formation, gentian violet dye, deletion

Abstract

In this study we examined the effect of bssS gene deletion, presumably involved in biofilm formation, on virulence factors productions in Erwinia amylovora. Compared to wild-type cells, E. amylovora E2 ∆bssS cells were characterised by reduced motility and biofilm formation on growth media without glucose. However, in the presence of glucose, the ability of deletion mutant cells to move and form biofilms was increased. In addition, it was shown that the cells of the mutant strain had increased sensitivity to the gentian violet dye.

Author Biographies

  • Karina Yu. Pesotskaya, Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus

    assistant at the department of molecular biology, faculty of biology

  • Alexander L. Lagonenko, Algimed Techno, 22/1 Lagojski Tract, Minsk 220090, Belarus

    PhD (biology), docent; head of the genetic and protein group

  • Anatoli N. Evtushenkov, Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus

    doctor of science (biology), full professor; professor at the department of molecular biology, faculty of biology

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Published

2024-11-16

Issue

No. 3 (2024): Experimental Biology and Biotechnology

Section

Genetics and Molecular Biology

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How to Cite

Phenotypic characteristics of bssS mutant of phytopathogenic bacteria Erwinia amylovora. (2024). Experimental Biology and Biotechnology, 3, 59-68. https://journals.bsu.by/index.php/biology/article/view/6098