Biophysical data on the influence of polychromatic light on the growth, phenotypic changes and protein metabolism of microalgae Chlorella vulgaris IBCE C-19

Authors

  • Ivan D. Klimovich Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus
  • Olga V. Nedzvedz Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus

Keywords:

Chlorella vulgaris, polychromatic light, photoreceptor, protein metabolism, microalgae biophysics, phenom
Supporting Agencies
The authors express their gratitude to E. M. Kabachevskaya, PhD ( biology), head of the laboratory of biophysics and biochemistry of plant cell of the Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus, for providing the algae strains and phytolamps, as well as for valuable advice and ideas that contributed to the successful completion of this study.

Abstract

The effect of polychromatic light on the growth, phenotypic changes and protein metabolism of microalgae Chlorella vulgaris IBCE C-19 has been studied. For the first time, spectrum-dependent biophysical effects have been systematically studied under conditions close to sunlight. It has been established that the blue (400–500 nm) and yellow-green (500–600 nm) spectral ranges stimulate protein synthesis, while red (600–700 nm) range has an inhibitory effect on it. The stimulative effect of near ultraviolet radiation (380–400 nm) was revealed, manifested in an increase in the number of cells and the specific growth rate of microalgae. An increase in the proportion of far-red light in the light spectrum (700–780 nm) correlates with a decrease in the intensity of RGB channels, which may indicate a change in the relative composition of pigments. The results of the work are of practical importance for optimising light modes in microalgae biotechnology.

Author Biographies

  • Ivan D. Klimovich, Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus

    student at the faculty of biology

  • Olga V. Nedzvedz, Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus

    PhD (engineering), docent; associate professor at the department of cell biology and plant bioengineering, faculty of biology

References

  1. Tamoi M, Shigeoka S. Diversity of regulatory mechanisms of photosynthetic carbon metabolism in plants and algae. Bioscience, Biotechnology, and Biochemistry. 2015;79(6):870 – 876. DOI: 10.1080/09168451.2015.1020754.
  2. Shi R, Dong G, Wang H, Chen F, Bi Y. A review on the optimization of light conditions in Chlorella culture. BIO Web of Conferences. 2024;142:02011. DOI: 10.1051/bioconf/202414202011.
  3. Zhu T, Guan G, Huang L, Wen L, Li L, Ren M. Transcriptomic and metabolomic analysis reveal the effects of light quality on the growth and lipid biosynthesis in Chlorella pyrenoidosa. Biomolecules. 2024;14(9):1144. DOI: 10.3390/biom14091144.
  4. Liu X, Hong Y, Gu W. Influence of light quality on Chlorella growth, photosynthetic pigments and high-valued products accumulation in coastal saline-alkali leachate. Water Reuse. 2021;11(2):301–311. DOI: 10.2166/wrd.2021.088.
  5. Kim DG, Lee C, Park S-M, Choi Y-E. Manipulation of light wavelength at appropriate growth stage to enhance biomass productivity and fatty acid methyl ester yield using Chlorella vulgaris. Bioresource Technology. 2014;159:240 –248. DOI: 10.1016/j.biortech.2014.02.078.
  6. del Pilar Sánchez-Saavedra M, Sauceda-Carvajal D, Castro-Ochoa FY, Molina-Cárdenas CA. The use of light spectra to improve the growth and lipid content of Chlorella vulgaris for biofuels production. Bioenergy Research. 2020;13(2):487– 498. DOI: 10.1007/s12155-019-10070-1.
  7. Kianianmomeni A, Hallmann A. Algal photoreceptors: in vivo functions and potential applications. Planta. 2014;239(1):1–26. DOI: 10.1007/s00425-013-1962-5.
  8. Meya G, Kowallik W. Involvement of two different photoreceptors in the light regulation of glutamine synthetase activity in a chlorophyll-free Chlorella mutant. Zeitschrift für Naturforschung C. 1994;49(11–12):757–762. DOI: 10.1515/znc-1994-11-1210.
  9. Fernández MB, Tossi V, Lamattina L, Cassia R. A comprehensive phylogeny reveals functional conservation of the UV-B photoreceptor UVR8 from green algae to higher plants. Frontiers in Plant Science. 2016;7:1698. DOI: 10.3389/fpls.2016.01698.
  10. Stöhr C, Glogau U, Mätschke M, Tischner R. Evidence for the involvement of plasma-membrane-bound nitrate reductase in signal transduction during blue-light stimulation of nitrate uptake in Chlorella saccharophila. Planta. 1995;197(4):613 – 618. DOI: 10.1007/BF00191568.
  11. Moriyama T, Terasawa K, Sekine K, Toyoshima M, Koike M, Fujiwara M, et al. Characterization of cell-cycle-driven and light-driven gene expression in a synchronous culture system in the unicellular rhodophyte Cyanidioschyzon merolae. Microbiology. 2010;156(6):1730 –1737. DOI: 10.1099/mic.0.037754-0.
  12. Olson BJSC. Assays for determination of protein concentration. Current Protocols in Pharmacology. 2016;73:A.3A.1–A.3A.32. DOI: 10.1002/cpph.3.
  13. Котеров АН, Ушенкова ЛН, Зубенкова ЭС, Калинина МВ, Бирюков АП, Ласточкина ЕМ и др. Сила связи. Сообщение 2. Градации величины корреляции. Медицинская радиология и радиационная безопасность. 2019;64(6):12–24. DOI: 10.12737/1024-6177-2019-64-6-12-24.
  14. Baidya A, Akter T, Islam MR, Shah AKMA, Hossain MA, Salam MA, et al. Effect of different wavelengths of LED light on the growth, chlorophyll, β-carotene content and proximate composition of Chlorella ellipsoidea. Heliyon. 2021;7(12):e08525. DOI: 10.1016/j.heliyon.2021.e08525.
  15. Holzinger A, Lütz C. Algae and UV irradiation: effects on ultrastructure and related metabolic functions. Micron. 2006;37(3):190 –207. DOI: 10.1016/j.micron.2005.10.015.
  16. Erofeeva EA. Plant hormesis: the energy aspect of low and high-dose stresses. Plant Stress. 2024;14:100628. DOI: 10.1016/j.stress.2024.100628.
  17. Beel B, Prager K, Spexard M, Sasso S, Weiss D, Müller N, et al. A flavin binding cryptochrome photoreceptor responds to both blue and red light in Chlamydomonas reinhardtii. The Plant Cell. 2012;24(7):2992–3008. DOI: 10.1105/tpc.112.098947.

Downloads

Additional Files

Published

2026-08-05

Issue

No. 1 (2026): Experimental Biology and Biotechnology

Section

Physiology and Сell Biology

License

Copyright (c) 2026 Experimental Biology and Biotechnology

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

The authors who are published in this journal agree to the following:

  1. The authors retain copyright on the work and provide the journal with the right of first publication of the work on condition of license Creative Commons Attribution-NonCommercial. 4.0 International (CC BY-NC 4.0).
  2. The authors retain the right to enter into certain contractual agreements relating to the non-exclusive distribution of the published version of the work (e.g. post it on the institutional repository, publication in the book), with the reference to its original publication in this journal.
  3. The authors have the right to post their work on the Internet (e.g. on the institutional store or personal website) prior to and during the review process, conducted by the journal, as this may lead to a productive discussion and a large number of references to this work. (See The Effect of Open Access.)

How to Cite

Klimovich, I. D., & Nedzvedz, O. V. (2026). Biophysical data on the influence of polychromatic light on the growth, phenotypic changes and protein metabolism of microalgae Chlorella vulgaris IBCE C-19. Experimental Biology and Biotechnology, 1, 24-32. https://doi.org/10.33581/2957-5060-2026-1-%p