The analysis of brassinosteroid effects on wheat root growth using phenotyping techniques

Authors

  • Vladislav U. Bondarenko Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus
  • Anatoliy I. Sokolik Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus
  • Alexey A. Vetoshkin Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus
  • Vladimir N. Zhabinsky Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus, acad. V. F. Kuprevich street, 5, building 2, 220141, Minsk, Republic of Belarus
  • Vladimir A. Khripach Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus, acad. V. F. Kuprevich street, 5, building 2, 220141, Minsk, Republic of Belarus
  • Vadzim V. Demidchik Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus

Keywords:

brassinosteroids, root growth and development, phenomic approach, wheat

Abstract

Growth reactions of wheat root on 10–12 –10–7 mol/l 24-epibrassinolide (EB), 28-homobrassinolide (HB) and 24-epicastasteron (EC) were examined. Wheat cultivar Vasilisa was used. All tested BRs demonstrated inhibitory action on wheat root length. Dose-dependence of the effect showed complex bell-like shape. Low BR levels induced reactions similar to highest BR levels. Concentrations of 10–10 –10–9 mol/l caused maximal inhibition of root growth rate. At the same time, root diameter significantly increased after BR treatment, as did mean roots hair length. So the volume of the root did not change significantly while their architecture was dramatically modified by BRs. Manually performed root growth measurements were compared with tests by phenotyping and demonstrated that the designed phenotyping techniques provided very accurate data. The root architecture phenotyping also yielded accurate results. Overall, phenotyping method showed that BRs inhibited wheat root growth and induced increase of root diameter and length of root hairs.

Author Biographies

  • Vladislav U. Bondarenko, Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus

    student at the faculty of biology

  • Anatoliy I. Sokolik, Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus

    docent; associate professor at the department of plant cell biology and bioengineering, faculty of biology

  • Alexey A. Vetoshkin, Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus

    student at the faculty of biology

  • Vladimir N. Zhabinsky, Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus, acad. V. F. Kuprevich street, 5, building 2, 220141, Minsk, Republic of Belarus

    doctor of science (chemistry), docent; chief researcher at the laboratory of steroid chemistry

  • Vladimir A. Khripach, Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus, acad. V. F. Kuprevich street, 5, building 2, 220141, Minsk, Republic of Belarus

    academician of the National Academy of Sciences of Belarus, doctor of science (chemistry), full professor; head of the laboratory of steroid chemistry.

  • Vadzim V. Demidchik, Belarusian State University, Nezavisimosti avenue, 4, 220030, Minsk, Republic of Belarus

    doctor of science (biology), docent; head of the department of plant cell biology and bioengineering, faculty of biology.

References

  1. Chinchilla D., Zipfel C., Robatzek S., et al. A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence. Nature. 2007. No. 448 (7152). P. 497–500. DOI: 10.1038/nature05999.
  2. Straltsova D., Chykun P., Subramaniam S., et al. Cation channels are involved in brassinosteroid signalling in higher plants. Steroids. 2015. Vol. 97, No. 5. P. 98–106.
  3. Khripach V. A., Zhabinskii V. N., Lakhvich F. A. Brassinosteroids. Minsk, 1993 (in Russ.).
  4. Pereira-Netto A. B. Brassinosteroids: Practical Applications in Agriculture and Human Health. New York, 2012. DOI: 10.2174/ 97816080529811120101.
  5. Dodd A. N., Kudla J., Sanders D. The Language of Calcium Signaling. Annual Review Plant Biol. 2010. Vol. 61. P. 593–620. DOI: 10.1146/annurev-arplant-070109-104628.
  6. Zullo M. A. T., Adam G. Brassinosteroid phytohormones – structure, bioactivity and applicatios. Brazilian J. Plant physiol. 2002. No. 14 (3). P. 143–181.
  7. Cheon J., Fujioka S., Dilkes B. P., et al. Brassinosteroids Regulate Plant Growth through Distinct Signaling Pathways in Selaginella and Arabidopsis. PLoS One. 2013. Vol. 8, issue 12. e81938. DOI: 10.1371/journal.pone.0081938.
  8. Prusakova L. D., Chizhova S. I., Khripach V. A. Resistance to lodging and yielding capacity of cereals under effect of brassinosteroids. Agricult. Biol. 1995. No. 1. P. 93 (in Russ.).
  9. Takematsu T., Ikekawa N., Shida A. Increasing the yield of cereals by means of brassinolide derivatives. US Patent. 1988. 4,767,442.
  10. Kim K. S., Sa J. G. Effects of plant growth regulator, brassinolide, on seedling growth in rice (Oryza sativa L.). Research Reports of Rural Development Administration (Suweon). 1989. Vol. 31 (1, Rice). P. 49–53.
  11. Singh J., Nakamura S., Ota Y. Effect of epibrassinolide on gram (Cicer arietinum) plants grown under water stress in juvenil stage. Indian J. Agricult. Sciences. 1993. Vol. 63. P. 395–397.
  12. Bajguz A., Hayat S. Effects of brassinosteroids on the plant responses to environmental stresses. Plant physiol. and Biochem. 2009. Vol. 47, No. 1. P. 1–8. DOI: 10.1016/j.plaphy.2008.10.002.
  13. Müssig C., Shin G.-H., Altmann T. Brassinosteroids Promote Root Growth in Arabidopsis. Plant Physiol. 2003. Vol. 133, No. 3. P. 1261–1271. DOI: 10.1104/pp.103.028662.

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Published

2017-12-03

Issue

No. 1 (2017): Journal of the Belarusian State University. Biology

Section

Physiology and Сell Biology

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How to Cite

Bondarenko, V. U., Sokolik, A. I., Vetoshkin, A. A., Zhabinsky, V. N., Khripach, V. A., & Demidchik, V. V. (2017). The analysis of brassinosteroid effects on wheat root growth using phenotyping techniques. Experimental Biology and Biotechnology, 1, 31-37. https://journals.bsu.by/index.php/biology/article/view/2425