Inhibitory effect of P2X7 receptor antagonists on neutrophil α-defensin-induced interleukin-8 production by human macrophages

Authors

  • Yury V. Chaly International Sakharov Environmental Institute of Belarusian State University, Daǔhabrodskaja Street, 23/1, 220070, Minsk, Belarus
  • Natallia N. Nashkevich International Sakharov Environmental Institute of Belarusian State University, Daǔhabrodskaja Street, 23/1, 220070, Minsk, Belarus

Keywords:

α-defensins, P2X7 receptor antagonists, macrophages, interleukin-8 (CXCL8)
Supporting Agencies
The work is supported by grants 03-55-2196 and 03-50-4211 IN

Abstract

Myeloid α-defensins are multifunctional cationic peptides of innate immune system that play an important role in infection, inflammation and human disease. We previously showed participation of HNP 1–3 (human neutrophil peptides 1–3) in regulation of inflammation by stimulation of monocytes and non-monocytic cells to express some cytokines, i. e. сhemokine interleukin-8 (IL-8, CXCL8), in response to HNP, but mechanisms of this induction retained unclear. Using P2X7 receptor antagonists PPADS and KN-62 an effective inhibition of HNP-dependent IL-8 induction in cultures of human macrophages was shown. In contrast to the effect of P2X7 antagonists on, TNFα-induced IL-8 induction was resistant to the action of KN-62. The data suggest the possible involvement of purinergic P2X7 receptor in cell effects of macrophages mediated by neutrophil-released HNP. Our data are useful for understanding of a role of НNР in inflammatory responses and pathological events, as well as for development of tools for neutralization of HNP-mediated detrimental cell effects and improving of diagnostic and therapy methods for HNP-dependent pathologies. 

Author Biographies

  • Yury V. Chaly, International Sakharov Environmental Institute of Belarusian State University, Daǔhabrodskaja Street, 23/1, 220070, Minsk, Belarus

    PhD (biology); research assistant professor of pediatrics – rheumatology, stead family department of pediatrics, Carver College of Medicine, University of Iowa

  • Natallia N. Nashkevich, International Sakharov Environmental Institute of Belarusian State University, Daǔhabrodskaja Street, 23/1, 220070, Minsk, Belarus

    PhD (biology); associate professor at the department of radiation hygiene and epidemiology

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Published

2017-12-16

Issue

No. 2 (2017): Journal of the Belarusian State University. Biology

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Physiology and Сell Biology

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How to Cite

Chaly, Y. V., & Nashkevich, N. N. (2017). Inhibitory effect of P2X7 receptor antagonists on neutrophil α-defensin-induced interleukin-8 production by human macrophages. Experimental Biology and Biotechnology, 2, 25-30. https://journals.bsu.by/index.php/biology/article/view/2441