Biosynthesis of ovine recombinant interferon-α in Еsсherichia  coli cells

Authors

  • Krystsina V. Vostrykava Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus
  • Maksim I. Patapovich Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus
  • Uladzimir A. Prakulevich Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

Keywords:

cloning, expression, ovine interferon-α, induction, recombinant protein, mass-spectrometric analysis

Abstract

Viral infection diseases cause huge losses to livestock. Although bacterial infections can be controlled by antibiotics, there is a lack of effective treatment for viral diseases. Recombinant interferons have broad spectrum of antiviral activity and can be used to solve this problem. As a result of the study the ovine interferon-α gene sequence was optimized for expression in gram-negative bacteria and cloned in Еscherichia coli cells as a part of the pET24b vector. A strain producing recombinant ovine interferon-α with a target protein yield of about 40 % of the total cellular protein was obtained. 

Author Biographies

  • Krystsina V. Vostrykava, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

    postgraduate student at the department of microbiology, faculty of biology

  • Maksim I. Patapovich, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

    head of the research laboratory of biotechnology at the department of microbiology, faculty of biology

  • Uladzimir A. Prakulevich, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

    doctor of science (biology), full professor; head of the department of microbiology, faculty of biology

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Published

2017-12-16

Issue

No. 2 (2017): Journal of the Belarusian State University. Biology

Section

Genetics, Microbiology and Molecular Biology

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How to Cite

Vostrykava, K. V., Patapovich, M. I., & Prakulevich, U. A. (2017). Biosynthesis of ovine recombinant interferon-α in Еsсherichia  coli cells. Experimental Biology and Biotechnology, 2, 43-50. https://journals.bsu.by/index.php/biology/article/view/2444