Morphogenesis of grape microplants in in vitro culture using biologically active substances of synthetic origin

Authors

  • Tatiana A. Krasinskaya Institute for fruit growing, 2 Kavaliova Street, Samachvalavičy 223013, Мinsk region, Belarus; International Sakharov Environmental Institute, Belarusian State University, 23 Daŭhabrodskaja Street, Minsk 220070, Belarus
  • Alexandr A. Zmushko Institute for fruit growing, 2 Kavaliova Street, Samachvalavičy 223013, Мinsk region, Belarus

Keywords:

Vitis L., 6-benzylaminopurine, thidiazuron, kinetin, in vitro culture, micropropagation of grape
Supporting Agencies
The work was performed with financial support of Belarusian Republican Foundation for Fundamental Research (project No. Б17-097).

Abstract

The aim of research was to study the influence of biologically active substances of synthetic origin (6-benzylaminopurine, thidiazuron and kinetin) on morphological development of microplants of cv. Agat donskoy and Mars on proliferation stage. Cv. Mars has the largest potential for gemmogenesis. On media with kinetin cv. Mars formed roots. On media with thidiazuron cv.Agat donskoy formed callus at base of conglomerate. Thidiazuron and kinetin on 4th subculture cause increase of percent of vitrified plants to 52.8–59.8 % depending on cultivar. During 4th and 5th subcultures modified MS culture with 1.1 and 1.5 mg /l BAP resulted in large number of normal developed microplants during gemmogenesis, without negative effects detected when using thidiazuron and kinetin. On average, for studied cultivars, use of modified MS culture
with 1.1 and 1.5 mg /l BAP promoted activation of gemmogenesis, propagation coefficient was 4.3 and 4.7.

Author Biographies

  • Tatiana A. Krasinskaya, Institute for fruit growing, 2 Kavaliova Street, Samachvalavičy 223013, Мinsk region, Belarus; International Sakharov Environmental Institute, Belarusian State University, 23 Daŭhabrodskaja Street, Minsk 220070, Belarus

    PhD (biology); senior researcher at the department of biotechnology,  Institute for fruit growing
    ; associate professor at the department of general biology, ecology and ecological genetics,  International Sakharov Environmental Institute

  • Alexandr A. Zmushko, Institute for fruit growing, 2 Kavaliova Street, Samachvalavičy 223013, Мinsk region, Belarus

    PhD (agricultural sciences); researcher at the department of biotechnology

References

  1. Bugaenko LO, Ivanova-Hanina LV. Morfogenez vinograda v kulʼture in vitro [Morphogenesis of grape in in vitro culture]. Uchenye zapiski Tavricheskogo natsionalʼnogo universiteta im. V. I. Vernadskogo. Seriya: Biologiya, khimiya. 2011;24(63-2):73–82 (in Russ.).
  2. Batukaev AA, Edieva H, Batukaev MS. Biotechnological methods of accelerated reproduction of grapes varieties. Nauchnye trudy GNU SKZNIISiV. 2013;1:271–275 (in Russ.).
  3. Kim S-H, Kim S-K. Effect of cytokinins on in vitro growth of grapes (Vitis spp.). Journal of Plant Biotechnology. 2002;29(2):123–127. DOI: 10.5010/JPB.2002.29.2.123.
  4. Gray DJ, Klein CM. In vitro shoot micropropagation and plant establishment of «Orlando Seedless» grape and «Tampa» rootstock. Proceedings of the Florida State Horticultural Society. 1987;100:308–309.
  5. Krasinskaya T, Kolbanova E. Grape viruses in Belarus. Acta horticulture. 2017;1188:307–311. DOI: 10.17660/ActaHortic.2017.1188.40.
  6. Enrico G, Imazio SA, Sgarbi E. In vitro propagation of Italian cultivars of Vitis vinifera and evaluation of genetic stability by SSRs markers. Acta horticulture. 2017;1155:165–172. DOI: 10.17660/ActaHortic.2017.1155.23.

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Published

2019-01-18

Issue

No. 2 (2018): Journal of the Belarusian State University. Biology

Section

Plant Cell Biology and Biotechnology

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How to Cite

Krasinskaya, T. A., & Zmushko, A. A. (2019). Morphogenesis of grape microplants in in vitro culture using biologically active substances of synthetic origin. Experimental Biology and Biotechnology, 2, 95-104. https://journals.bsu.by/index.php/biology/article/view/2513