Cell responses to the impact of physiological doses of ultraviolet radiation and their modulation by plant polyphenols

Authors

  • Vladimir A. Kostyuk Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Ahmed Albuhaydar Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Alla I. Potapovich Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Lyudmila G. Korkinа Centre for Innovative Biotechnological Investigations «Nanolab», 3 Odoevsky Passage, 7 building, Moscow 117574, Russia

Keywords:

ultraviolet radiation, plant polyphenolic compounds, inflammatory mediators, keratinocytes, fibroblasts

Abstract

In this study it was investigated the responses of cultured human cells – keratinocytes and fibroblasts to physiological doses of ultraviolet radiation (UVR) applied with or without plant polyphenolic compounds: rutin, quercetin, taxofolin, silybin and baikalein. Experimental data obtained in this work indicate the presence of synergism in the action of physiological doses of UVR and plant polyphenolic compounds on inflammatory signaling pathways in keratinocytes and fibroblasts. It is concluded that the identified synergies can contribute to the adaptation of the skin to subsequent exposure to UVR, and thus be one of the photoprotective mechanisms of the plant polyphenolic compounds.

Author Biographies

  • Vladimir A. Kostyuk, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    doctor of science (chemistry), docent; head of the research laboratory of physiology, department of human and animal physiology, faculty of biology

  • Ahmed Albuhaydar, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    postgraduate student at the department of human and animal physiology, faculty of biology

  • Alla I. Potapovich, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    PhD (biology); leading researcher at the research laboratory of physiology, department of human and animal physiology, faculty of biology

  • Lyudmila G. Korkinа, Centre for Innovative Biotechnological Investigations «Nanolab», 3 Odoevsky Passage, 7 building, Moscow 117574, Russia

    doctor of science (medicine); full professor

References

  1. Scharffetter-Kochanek K, Wlaschek M, Brenneisen P, Schauen M, Blaudschun R, Wenk J. UV-induced reactive oxygen species in photocarcinogenesis and photoaging. Journal of Biological Chemistry. 1997;378(11):1247–1257.
  2. Fisher GJ, Wang Z, Datta SC, Varani J, Kang S, Voorhees JJ. Pathophysiology of premature skin aging induced by ultraviolet light. The New England Journal of Medicine. 1997;337:1419–1428. DOI: 10.1056/NEJM199711133372003.
  3. Bode AM, Dong Z. Signal transduction pathways in cancer development and as targets for cancer prevention. Progress in Nucleic Acid Research and Molecular Biology. 2005;79:237–297. DOI: 10.1016/S0079-6603(04)79005-4.
  4. Valencia A, Kochevar IE. Nox1-based NADPH oxidase is the major source of UVA-induced reactive oxygen species in human keratinocytes. Journal of Investigative Dermatology. 2008;128:214–222. DOI: 10.1038/sj.jid.5700960.
  5. Peus D, Vasa RA, Meves A, Pott M, Beyerle A, Squillace K, Pittelkow MR. H2O2 is an important mediator of UVB induced EGF-receptor phosphorylation in cultured keratinocytes. Journal of Investigative Dermatology. 1998;110:966 – 971. DOI: 10.1046/j.1523-1747.1998.00210.x.
  6. Mahns A, Wolber R, Stab F, Klotz LO, Sies H. Contribution of UVB and UVA to UV-dependent stimulation of cyclooxygenase-2 expression in artificial epidermis. Photochemical and Photobiological Sciences. 2004;3:257–262. DOI: 10.1039/b309067a.
  7. Adams DO, Hamilton TA. Macrophages as destructive cells in host defense. In: Gallin JI, Goldstein IM, Synderman R, editors. Inflammation: basic principles and clinical correlates. New York: Raven Press; 1992. p. 637–662.
  8. Bruls WA, van Weelden H, van der Leun JC. Transimission of UV-radiation through human epidermal layers as a factor influencing the minimal erythema dose. Photochemistry and Photobiology. 1984;39:63–67.
  9. Hebb TP. Skin diseases. Diagnosis and treatment. 3rd edition. New York: Elsevior; 2005. Russian edition: Khebif TP. Kozhnye bolezni. Diagnostika i lechenie. Kubanova AA, editor. Moscow: MEDpress inform; 2008.
  10. Bonamigo RR, Dornelles SIT, editors. Dermatology in Public Health Environments: A Comprehensive Textbook. New York: Springer; 2018.
  11. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative pcr and the 2(-delta delta c(t)) method. Methods. 2001;25:402– 408.
  12. Kostyuk VA, Potapovich AI. Superoxide-driven oxidation of quercetin and a simple sensitive assay for determination of superoxide dismutase. Biochemistry International. 1989;19:1117–1124.
  13. Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases: The first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry. 1974;249:7130 –7139.
  14. Tyurin VA, Tyurina YY, Borisenko GG, Sokolova TV, Ritov VB, Quinn PJ, et al. Oxidative stress following traumatic brain injury in rats: quantitation of biomarkers and detection of free radical intermediates. Journal of Neurochemistry. 2000;75(5):2178–2189.
  15. Korkina LG, Pastore S, De Luca C, Kostyuk VA. Metabolism of plant polyphenols in the skin: beneficial versus deleterious effects. Current Drug Metabolism. 2008;9:710 –729.
  16. Potapovich AI, Kostyuk VA, Kostyuk TV, De Luca C, Korkina LG. Effects of pre- and post-treatment with plant polyphenols on human keratinocyte responses to solar UV. Inflammation Research. 2013;62(8):773–780.
  17. He YY, Council SE, Feng L, Chignell CF. UVA-induced cell cycle progression is mediated by a disintegrin and metalloprotease/epidermal growth factor receptor/AKT/Cyclin D1 pathways in keratinocytes. Cancer Research. 2008;68:3752–3758.
  18. Meeran SM, Katiyar N, Singh T, Katiyar SK. Loss of endogenous interleukin-12 activates survival signals in ultraviolet-exposed mouse skin and skin tumors. Neoplasia. 2009;11:846 – 855.
  19. Baumann H, Gauldie J. The acute phase response. Immunology Today. 1994;15:74 – 80.

Downloads

Additional Files

Published

2019-07-01

Issue

No. 2 (2019): Journal of the Belarusian State University. Biology

Section

Cell Biology and Physiology

License

The authors who are published in this journal agree to the following:

  1. The authors retain copyright on the work and provide the journal with the right of first publication of the work on condition of license Creative Commons Attribution-NonCommercial. 4.0 International (CC BY-NC 4.0).
  2. The authors retain the right to enter into certain contractual agreements relating to the non-exclusive distribution of the published version of the work (e.g. post it on the institutional repository, publication in the book), with the reference to its original publication in this journal.
  3. The authors have the right to post their work on the Internet (e.g. on the institutional store or personal website) prior to and during the review process, conducted by the journal, as this may lead to a productive discussion and a large number of references to this work. (See The Effect of Open Access.)

How to Cite

Kostyuk, V. A., Albuhaydar, A. ., Potapovich, A. I., & Korkinа L. G. (2019). Cell responses to the impact of physiological doses of ultraviolet radiation and their modulation by plant polyphenols. Experimental Biology and Biotechnology, 2, 13-21. https://doi.org/10.33581/2521-1722-2019-2-13-21