Comment of the authors on the article «The contribution of various mechanisms to mRNA diversity of human fusion oncogene RUNX1-RUNX1T1»

Authors

  • Ilya M. Ilyushonak Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus https://orcid.org/0000-0002-5377-366X
  • Lev I. Polkhovsky Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Vasily V. Grinev Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

Keywords:

fusion oncogene RUNX1-RUNX1T1, alternative RNA splicing, high throughput sequencing, biological noise
Supporting Agencies
This work is a part of sub-programme «Union» of the State Program on Scientific Research «Convergence-2020» (grant 3.08.03 (No. 469/54)).

Abstract

The main topic of this brief communication is the critical review of flaws, found in the original article «The contribution of various mechanisms to mRNA diversity of human fusion oncogene RUNX1-RUNX1T1», which was published in «Journal of the Belarusian State University. Biology» (2019, No. 2). Also authors provide new results obtained since publication.

Author Biographies

  • Ilya M. Ilyushonak, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    assistant at the department of genetics, faculty of biology

  • Lev I. Polkhovsky, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    student at the faculty of biology

  • Vasily V. Grinev, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    PhD (biology), docent; associate professor at the department of genetics, faculty of biology

References

  1. Ilyushonak IM, Migas AA, Sukhareuski AY, Schneider AD, Grinev VV. The contribution of various mechanisms to mRNA diversity of human fusion oncogene RUNX1-RUNX1T1. Journal of the Belarusian State University. Biology. 2019;2:45–59. Russian. DOI: 10.33581/2521-1722-2019-2-45-59.
  2. Ishqi HM, Husain MA, Rehman SU, Sarwar T, Tabish M. Identification and expression of alternatively spliced novel isoforms of cancer associated MYD88 lacking death domain in mouse. Molecular Biology Reports. 2018;45(5):699–711. DOI: 10.1007/s11033-018-4209-5.
  3. Houseley J, Tollervey D. Apparent non-canonical trans-splicing is generated by reverse transcriptase in vitro. PLOS ONE [Internet]. 2010 [cited 2020 April 20];5(8):e12271. Available from: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0012271. DOI: 10.1371/journal.pone.0012271.
  4. Mader RM, Schmidt WM, Sedivy R, Rizovski B, Braun J, Kalipciyan M, et al. Reverse transcriptase template switching during reverse transcriptase – polymerase chain reaction: artificial generation of deletions in ribonucleotide reductase mRNA. Translational Research. The Journal of Laboratory and Clinical Medicine. 2001;137(6):422–428. DOI: 10.1067/mlc.2001.115452.
  5. Delviks KA, Pathak VK. Effect of distance between homologous sequences and 3′ homology on the frequency of retroviral reverse transcriptase template switching. Journal of Virology. 1999;73(10):7923–7932. DOI: 10.1128/JVI.73.10.7923-7932.1999.
  6. Cariello NF, Thilly WG, Swenberg JA, Skopek TR. Deletion mutagenesis during polymerase chain reaction: dependence on DNA polymerase. Gene. 1991;99(1):105–108. DOI: 10.1016/0378-1119(91)90040-I.
  7. Markham NR, Zuker M. DINAMelt web server for nucleic acid melting prediction. Nucleic Acids Research. 2005;33(suppl_2):W577–W581. DOI: 10.1093/nar/gki591.
  8. Birol I, Raymond A, Chiu R, Nip KM, Jackman SD, Kreitzman M, et al. KLEAT: cleavage site analysis of transcriptomes. In: Altman RB, Dunker AK, Hunter L, Ritchie MD, Murray T, Klein TE, editors. Pacific symposium on biocomputing 2015; 4–8 January 2015; Kohala Coast, Hawaii, USA. New Jersey: World Scientific; 2015. p. 347–358.
  9. Arefeen A, Liu J, Xiao X, Jiang T. TAPAS: tool for alternative polyadenylation site analysis. Bioinformatics. 2018;34(15):2521–2529. DOI: 10.1093/bioinformatics/bty110.

Downloads

Additional Files

Published

2020-09-12

Issue

No. 2 (2020): Journal of the Belarusian State University. Biology

Section

Short Communications

License

The authors who are published in this journal agree to the following:

  1. The authors retain copyright on the work and provide the journal with the right of first publication of the work on condition of license Creative Commons Attribution-NonCommercial. 4.0 International (CC BY-NC 4.0).
  2. The authors retain the right to enter into certain contractual agreements relating to the non-exclusive distribution of the published version of the work (e.g. post it on the institutional repository, publication in the book), with the reference to its original publication in this journal.
  3. The authors have the right to post their work on the Internet (e.g. on the institutional store or personal website) prior to and during the review process, conducted by the journal, as this may lead to a productive discussion and a large number of references to this work. (See The Effect of Open Access.)

How to Cite

Ilyushonak, I. M. ., Polkhovsky, L. I. ., & Grinev, V. V. . (2020). Comment of the authors on the article «The contribution of various mechanisms to mRNA diversity of human fusion oncogene RUNX1-RUNX1T1». Experimental Biology and Biotechnology, 2, 90-94. https://doi.org/10.33581/2521-1722-2020-2-90-94