Characteristic of strains Priestia megaterium Ср-1 and Rhodococcus jostii СА-6 demonstrating increased salt resistance

Authors

  • Nadezhda I. Naumovich Institute of Microbiology, National Academy of Sciences of Belarus, 2 Akademika Kupreviča Street, Minsk 220141, Belarus
  • Zinaida M. Aleschenkova Institute of Microbiology, National Academy of Sciences of Belarus, 2 Akademika Kupreviča Street, Minsk 220141, Belarus
  • Irina N. Ananyeva Institute of Microbiology, National Academy of Sciences of Belarus, 2 Akademika Kupreviča Street, Minsk 220141, Belarus
  • Halina V. Safronava Institute of Microbiology, National Academy of Sciences of Belarus, 2 Akademika Kupreviča Street, Minsk 220141, Belarus

Keywords:

nitrogen fixation, phosphate solubilisation, growth stimulation, osmolytes, salt-induced stress

Abstract

The strains Priestia megaterium Ср-1 and Rhodococcus jostii CA-6 were shown to preserve phosphate-solubilising ability, nitrogen-fixing activity and synthesis of indole-3-acetic acid under osmotic stress conditions caused by sodium chloride in concentration range of 1197–2052 mmol/L, as well as to promote seed germination rate and development of small radish seedlings. Proline, betaine and sucrose were found to exert a beneficial effect on growth and ability of strains P. megaterium Ср-1 and Rh. jostii CA-6 to adapt to osmotic stress. Mass spectrometric analysis has revealed that intracellular synthesis of proline and betaine in both bacteria was directly proportional to sodium chloride concentration: rising NaCl level in the medium to 1197 mmol/L was accompanied by increase in proline level from 0.062 to 1.502 µg /mL (Rh. jostii CA-6) and from 0.089 to 2.105 µg /mL (P. megaterium Ср-1), in a similar manner betaine production grew up from 0.071 to 0.118 µg /mL (Rh. jostii СА-6) and from 0.055 to 0.219 µg /mL (P. megaterium Ср-1).

Author Biographies

  • Nadezhda I. Naumovich, Institute of Microbiology, National Academy of Sciences of Belarus, 2 Akademika Kupreviča Street, Minsk 220141, Belarus

    researcher at the laboratory of interactions between soil microorganisms and higher plants

  • Zinaida M. Aleschenkova, Institute of Microbiology, National Academy of Sciences of Belarus, 2 Akademika Kupreviča Street, Minsk 220141, Belarus

    doctor of science (biology); chief researcher at the laboratory of interactions between soil microorganisms and higher plants

  • Irina N. Ananyeva, Institute of Microbiology, National Academy of Sciences of Belarus, 2 Akademika Kupreviča Street, Minsk 220141, Belarus

    PhD (biology), docent; head of the laboratory of interactions between soil microorganisms and higher plants

  • Halina V. Safronava, Institute of Microbiology, National Academy of Sciences of Belarus, 2 Akademika Kupreviča Street, Minsk 220141, Belarus

    PhD (biology), docent; leading researcher at the laboratory of interactions between soil microorganisms and higher plants

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Published

2022-06-29

Issue

No. 2 (2022): Experimental Biology and Biotechnology

Section

Biotechnology and Microbiology

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How to Cite

Naumovich, N. I., Aleschenkova, Z. M., Ananyeva, I. N., & Safronava, H. V. (2022). Characteristic of strains Priestia megaterium Ср-1 and Rhodococcus jostii СА-6 demonstrating increased salt resistance. Experimental Biology and Biotechnology, 2, 60-72. https://doi.org/10.33581/2957-5060-2022-2-60-72