Interaction of nitrobenzoxadiazole derivatives of piperazine and aniline with bovine serum albumine in silico and in vitro

Authors

  • Yaroslav V. Faletrov Research Institute for Physical Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus; Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus https://orcid.org/0000-0001-8168-5897
  • Veronika S. Karpushenkova Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Viktoryia A. Zavalinich Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Polina S. Yakovets Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Anastasia D. Shkredava Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Vladimir M. Shkumatov Research Institute for Physical Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus; Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

Keywords:

bovine serum albumin, fluorescence, nitrobenzoxadiazoles, docking
Supporting Agencies
The work was supported by governmental program of scientific researches «Chemical processes, reagents and technologies, bioregulators and bioorgchemistry» (reg. No. 20210560).

Abstract

Albumin is a globular protein of plasma of mammalian blood participating in transport of hydrophobic metabolites and drugs. Thus, studies devoted to its binding are valuable as a part of evaluation of new potential drugs or fluorescent probes for in vivo usage. Here we describe results concerning synthesis and bovine serum albumin binding assay both in silico (docking) and in vitro (spectrophotometric and spectrofluorimetric titrations) for four new 7-nitrobenzoxadiazol-4-yl (NBD) derivatives of aniline and piperazine. Experimental dissociation constant for NBD-ethynylaniline 4 was calculated to be about 10 µmol/L.

Author Biographies

  • Yaroslav V. Faletrov, Research Institute for Physical Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus; Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    PhD (chemistry), docent; leading researcher at the laboratory of biochemistry of drugs, Research Institute for Physical Chemical Problems, Belarusian State University, and associate professor at the department of macromolecular compounds, faculty of chemistry, Belarusian State University

  • Veronika S. Karpushenkova, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    student at the faculty of chemistry

  • Viktoryia A. Zavalinich, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    student at the faculty of chemistry

  • Polina S. Yakovets, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    student at the faculty of chemistry

  • Anastasia D. Shkredava, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    student at the faculty of chemistry

  • Vladimir M. Shkumatov, Research Institute for Physical Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus; Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    corresponding member of the National Academy of Sciences of Belarus, doctor of science (biology), full professor; chief researcher at the laboratory of biochemistry of drugs, Research Institute for Physical Chemical Problems, Belarusian State University, and professor at the department of macromolecular compounds, faculty of chemistry, Belarusian State University

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Published

2021-09-22

Issue

No. 2 (2021): Journal of the Belarusian State University. Chemistry

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Original Articles

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How to Cite

[1]
Faletrov, Y.V. et al. 2021. Interaction of nitrobenzoxadiazole derivatives of piperazine and aniline with bovine serum albumine in silico and in vitro. Journal of the Belarusian State University. Chemistry. 2 (Sep. 2021), 25–35. DOI:https://doi.org/10.33581/2520-257X-2021-2-25-35.