Fluorescent properties and protein-ligand interactions of boron dipyrromethene molecular rotor

Authors

  • Anastasiya Dudko Research Institute for Physical Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus https://orcid.org/0000-0002-9961-5587
  • Matvey S. Horetski Research Institute for Physical Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus https://orcid.org/0000-0003-3065-6214
  • Nina S. Frolova Research Institute for Physical Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus
  • Yaroslav V. Faletrov Research Institute for Physical Chemical Problems, Belarusian State University; 14 Lieninhradskaja Street, Minsk 220006, Belarus; Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Vladimir M. Shkumatov Research Institute for Physical Chemical Problems of the Belarusian State University, 14, Leningradskaya str., 220030 Minsk, Republic of Belarus

Keywords:

бордипиррометен, бычий сывороточный альбумин, молекулярный докинг, молекулярный ротор, флуоресценция
Supporting Agencies
The work was supported by Belarusian Republican Foundation for Fundamental Research (grant No. Х21М-059) and governmental program of scientific researches «Chemical technologies and materials».

Abstract

A fluorescent molecular rotor (FMR) based on boron dipyrromethene core was obtained. Fluorescence emission properties of the compound strongly depend on the medium viscosity. The emission intensity increases 27 times after viscosity changing from ethanol to glycerol medium. At the same time, the compound appeared almost insensitive to solvatochromic effects. Obtained FMR also can affinely bind with the bovine serum albumin protein active sites followed by noticeable emission intensity growth. Possible ligand binding cites and main amino acid surrounding were described with molecular docking simulations and are in an agreement with experimental data. The results can be used for further design and synthesis of novel fluorescent viscosity and protein-ligand interactions censors suitable for implementation in biosystems in vitro and in vivo.

Author Biographies

  • Anastasiya Dudko, Research Institute for Physical Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus

    junior researcher trainee at the laboratory of biochemistry of drugs

  • Matvey S. Horetski, Research Institute for Physical Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus

    junior researcher at the laboratory of biochemistry of drugs

  • Nina S. Frolova, Research Institute for Physical Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus

    researcher at the laboratory of biochemistry of drugs

  • Yaroslav V. Faletrov, Research Institute for Physical Chemical Problems, Belarusian State University; 14 Lieninhradskaja Street, Minsk 220006, Belarus; Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

    PhD (chemistry), docent; leading researcher at the laboratory of biochemistry of drugs, Research Institute for Physical Chemical Problems, Belarusian State University, and associate professor at the department of macromolecular compounds, faculty of chemistry, Belarusian State University

  • Vladimir M. Shkumatov, Research Institute for Physical Chemical Problems of the Belarusian State University, 14, Leningradskaya str., 220030 Minsk, Republic of Belarus

    corresponding member of the National Academy of Sciences of Belarus, doctor of science (biology), full professor; professor at the department of macromolecular compounds, faculty of chemistry, Belarusian State University, and chief researcher at the laboratory of biochemistry of drugs, Research Institute for Physical Chemical Problems, Belarusian State University

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Published

2022-04-07

Issue

No. 1 (2022): Journal of the Belarusian State University. Chemistry

Section

Original Articles

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How to Cite

[1]
Dudko, A. et al. 2022. Fluorescent properties and protein-ligand interactions of boron dipyrromethene molecular rotor. Journal of the Belarusian State University. Chemistry. 1 (Apr. 2022), 61–71. DOI:https://doi.org/10.33581/2520-257X-2022-1-61-71.