Yeast fungi as promising agents for biocontrol of mycopathogens in grapes

  • Natalia N. Volynchuk Polessky State University, 23 Dniaprowskaj flacilii Street, Pinsk 225710, Brest Region, Belarus
  • Lyudmila F. Kabashnikova Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, 27 Akademichnaja Street, Minsk 220072, Belarus

Abstract

From the episphere and endosphere of various organs of cultivated grapes (Vitis vinifera L.) variety Alpha, 77 strains of yeast fungi were isolated, of which 6 strains after secondary screening showed antagonistic activity against postharvest mycopathogens of grapes Botrytis cinerea BIM F-71 and Fusarium oxysporum BIM F-609G. The average rate of inhibition of the mycelium of B. cinerea BIM F-71 by native yeast bioagents was 75.1 %, the average rate of inhibition of the mycelium of F. oxysporum BIM F-609G – 65.3 %. Analysis of the minimum inhibitory concentration showed that the concentration of 105 CFU/mL is sufficient to reduce the development of both pathogens by all yeast strains. Of the studied mechanisms of the antagonistic action of yeast fungi, there was a synthesis of urease, cellulase, amylase and partially protease. Production of volatile organic compounds by non-filamentous fungi against B. cinerea BIM F-71 and F. oxysporum BIM F-609G was not revealed.

Author Biographies

Natalia N. Volynchuk, Polessky State University, 23 Dniaprowskaj flacilii Street, Pinsk 225710, Brest Region, Belarus

postgraduate student at the department of biotechnology, biotechnological faculty

Lyudmila F. Kabashnikova, Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, 27 Akademichnaja Street, Minsk 220072, Belarus

doctor of science (biology), corresponding member of the National Academy of Sciences of Belarus, docent; head of the laboratory of applied biophysics and biochemistry

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Published
2023-11-08
Keywords: grapes, Botrytis, Fusarium, yeast fungi, biocontrol
How to Cite
Volynchuk, N. N., & Kabashnikova, L. F. (2023). Yeast fungi as promising agents for biocontrol of mycopathogens in grapes. Experimental Biology and Biotechnology, 3, 24-33. Retrieved from https://journals.bsu.by/index.php/biology/article/view/5657
Issue
No 3 (2023): Experimental Biology and Biotechnology
Section
Physiology and Сell Biology

Copyright (c) 2023 Experimental Biology and Biotechnology

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