The effectiveness of the use of recombinant lactoferrin for correcting biochemical irregularities in rats with experimental doxycycline-induced cholestasis and alloxan model of diabetes

Authors

  • Nataliya M. Oryol Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

Keywords:

lactoferrin, experimental cholestasis, an experimental diabetes mellitus, enzymes, lipid peroxidation

Abstract

It is experimentally shown that recombinant lactoferrin, when administered for 5 days in a dose of 40 mg/kg, does not change the activity index of aminotransferases, the enzymes of antioxidant defense, and the intensity of lipid peroxidation processes. The data suggest that its entry into an intact organism is practically harmless. Using the model of an experimental insulin dependent diabetes mellitus, it is established that lactoferrin in the studied doses normalizes the catalase activity, weakens the shifts in activity of aspartate aminotransferase in the blood serum, superoxide dismutase and lipid peroxidation processes in the liver and kidneys of rats, which develop with the administration of alloxan in a single dose of 150 mg/kg. In the next series of experiments, it was determined that lactoferrin is able to reliably decrease the negative changes in the activity of aminotransferases, lactate dehydrogenase, the enzymes of antioxidant defense, the processes of lipid peroxidation and lipid metabolism in rats with experimental cholestasis induced by administration of doxycycline for 5 days in a dose of 540 mg/kg of animal weight. 

Author Biography

  • Nataliya M. Oryol, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

    PhD (biology), docent; associate professor at the department of biochemistry, faculty of biology

References

  1. Borzenkova N. V., Balabushevich N. G., Larionova N. I. Lactoferrin: physical and chemical properties, biological functions, delivery systems, pharmaceutical and nutraceutical preparations. Russ. J. Biofarm. 2010. Vol. 2, No. 3. P. 3–19 (in Russ.).
  2. Krot I. F. Lactoferrin’s structure and functions and its possible application in obstetrics. Problems health ecol. 2005. No. 1. P. 65– 68 (in Russ.).
  3. Nikolaev A. A., Anshakova N. I. [Immunochemical and physico-chemical characterization of lactoferrin in human biological fluids]. Vopr. med. khim. 1985. No. 3. P. 128–131 (in Russ.).
  4. Gonzalez-Chavez S. A., Arevalo-Gallegos S., Rascon-Cruz Q. Lactoferrin: structure, function and applications. Int. J. Antimicrob. Agents. 2009. Vol. 33, issue 4. P. 301e1–301e.8. DOI: 10.1016/j.ijantimicag.2008.07.020.
  5. Jenssen H., Hancock R. E. Antimicrobial properties of lactoferrin. Biochimie. 2009. Vol. 91, issue 1. P. 19–29. DOI: 10.1016/j. biochi.2008.05.015.
  6. Lupetti A., Paulusma-Annema A., Welling M. M., et al. Synergistic activity of the N-terminal peptide of human lactoferrin and fluconazol against Candida species. Antimicrob. Agents Chemother. 2003. Vol. 47, issue 1. P. 262–267. DOI: 10.1128/AAC.47.1.262267.2003.
  7. Naot D., Grey A., Reid I. R., et al. Lactoferrin – a novel bone growth factor. Сlin. Med. Res. 2005. Vol. 3, issue 2. P. 93–101.
  8. Nozaki A., Ikeda M., Naganuma A., et al. Identification of a lactoferrin-derived peptide possessing binding activity to hepatitis C virus E2 envelope protein. J. Biol. Chem. 2003. Vol. 278, No. 12. P. 10162–10173. DOI: 10.1074/jbc.M207879200.
  9. Orsi N. The antimicrobial activity of lactoferrin: Current status and perspectives. Biometals. 2004. Vol. 17, issue 3. P. 189 –196. DOI: 10.1023/B:BIOM.0000027691.86757.e2.
  10. Welsh K. J., Hwang S. A., Boyd S., et al. Influence of oral lactoferrin on Mycobacterium tuberculosis induced immunopathology. Tuberculosis. 2011. Vol. 91, suppl. 1. P. 105–113. DOI: 10.1016/j.tube.2011.10.019.
  11. Gubskii K. I. Korrektsiya khimicheskogo porazheniya pecheni. Kyiv, 1989 (in Russ.).
  12. Golikov S. N., Sanotski I. V., Tiunov L. A. Obshchie mekhanizmy toksicheskogo deistviya. Leningrad, 1986 (in Russ.).
  13. Baranov V. G. (ed.) Eksperimentalnyi sakharnyi diabet. Leningrad, 1983 (in Russ.).
  14. Kamyshnikov V. S. Kliniko-biokhimicheskaya laboratornaya diagnostika : in 2 vol. Minsk, 2003 (in Russ.).
  15. Kostyuk V. A., Potapovich A. I., Kovaleva Zn. V. A simple, sensitive assay for determinination of superoxide dismutase activity, based on reaction of quercetin oxidation. Vopr. med. khim. 1990. Vol. 36, issue 2. P. 88 –91 (in Russ.).
  16. Korolyuk M. A., Ivanova L. I., Mayorova I. G., et al. [The method for determining the activity of catalase]. Lab. delo. 1988. No. 1. P. 16 –19 (in Russ.).
  17. Stal’naya I. D., Garishvili T. G. [Method for determination of malondialdehyde via thiobarbituric acid]. Sovremennye metody v biokhimii. Moscow, 1977. P. 66 – 68 (in Russ.).
  18. Peterson G. L. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal. Biochem. 1977. Vol. 83, issue 2. P. 346 –356. DOI:10.1016/0003-2697(77)90043-4.
  19. Lowry O. H., Rosebrough N. J., Farr A. L., et al. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951. Vol. 193, issue 1. P. 265–275.
  20. Rokitskii P. F. Biologicheskaya statistika. Minsk, 1967 (in Russ.).
  21. Sherlok S., Duli J. Zabolevaniya pecheni i zhelchnykh putei. Moscow, 1999 (in Russ.).
  22. Yakovenko E. P., Grigor’ev P. Y. Chronic liver disease: diagnosis and treatment. Russ. med. zh. 2003. Vol. 11, No. 5. P. 291–296 (in Russ.).
  23. Klimov A. N., Nikul’cheva N. G. Obmen lipidov i lipoproteidov i ego narusheniya. Saint-Petersburg, 1999 (in Russ.).
  24. Grigor’ev P. Y., Yakovenko E. P. Vnutripechenochnyi kholestaz pri boleznyakh pecheni: ot diagnoza do lecheniya. Lechashchii vrach. 1999. No. 6. P. 14 –17 (in Russ.).
  25. Batvinkov N. I., Garelik P. V. Mekhanicheskaya zheltukha: diagnostika i lechenie. Grodno, 2001 (in Russ.).
  26. Navashin P. S. Doksitsiklin: pokazaniya k primeneniyu i ikh farmakokineticheskie osnovy. Antibiotiki i khimioterapiya. 1991. Vol. 36, No. 1. P. 48 –51 (in Russ.).
  27. Oryol N. M., Pyshko Y. S., Sokolovskii D. Y., et al. [The regulation of metabolism in the liver of rats with experimental cholestasis by laser radiation on biologically active points]. Lazernaya fizika i opticheskie tekhnologii : materialy IX Mezhdunar. nauchn. konf. (Grodno, 30 May – 2 June, 2012) : in 2 parts. Grodno, 2012. Part 1. P. 118 –120 (in Russ.).
  28. Oryol N. M., Pyshko Y. S., Lisenkova A. M., et al. Vozdeistvie nizkointensivnym lazernym izlucheniem na oblast’ biologicheski aktivnykh tochek dlya korrektsii biokhimicheskikh narushenii v pecheni krys s eksperimental’nym vnutripechenochnym kholestazom. Lazery. Izmereniya. Informatsiya : materialy Mezhdunar. nauchn. konf. (Saint-Petersburg, 5–7 June, 2011) Saint-Petersburg, 2012. P. 65– 66 (in Russ.).
  29. Balabolkin M. I. Sakharnyi diabet: 100 voprosov i otvetov. Moscow, 1992 (in Russ.).
  30. Golovkin B. N., Rudenskaya I. A., Trofimova I. A., et al. Biologicheski aktivnye veshchestva rastitel’nogo proiskhozhdeniya : in 3 vol. Moscow, 2001. Vol. 1 (in Russ.).
  31. Shul’pekova Y. O. [Milk thistle flavonoids in the treatment of liver diseases]. Russ. med. zh. 2004. No. 5. P. 248 (in Russ.).
  32. Vengerovskii A. I., Saratikov A. S. [Action mechanisms of hepatoptrotectors under toxic liver damage]. Farmakologiya i toksikologiya. 1988. No. 1. P. 89 –93 (in Russ.).
  33. Actor J. K., Hwang S. A., Kuzel M. L. Lactoferrin as a natural immune modulator. Curr. Pharm. Des. 2009. Vol. 15, issue 17. P. 1956 –1973.
  34. Zharskaya A. V., Chumachenko M. S., Korik Ey. O. Studies of potential dermatotropic effects of flag cell extract and lactoferrin in model of induced contact dermatitis. Currernt problems in biochemistry : sb. nauchn. statei (Grodno, 5–6 July, 2016). Grodno, 2016. Part 1. P. 82– 88 (in Russ.).
  35. Hardwick R. N., Fisher C. D., Canet M. J., et al. Diversity in antioxidant response enzymes in progressive stages of human nonalcoholic fatty liver disease. Drug Metab. Dispos. 2010. Vol. 38, issue 12. P. 2293–2301. DOI: 10.1124/dmd.110.035006.

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Published

2017-12-16

Issue

No. 2 (2017): Journal of the Belarusian State University. Biology

Section

Biochemistry

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How to Cite

Oryol, N. M. (2017). The effectiveness of the use of recombinant lactoferrin for correcting biochemical irregularities in rats with experimental doxycycline-induced cholestasis and alloxan model of diabetes. Experimental Biology and Biotechnology, 2, 72-79. https://journals.bsu.by/index.php/biology/article/view/2448