The analysis of brassinosteroid effects on wheat root growth using phenotyping techniques
Abstract
Growth reactions of wheat root on 10–12 –10–7 mol/l 24-epibrassinolide (EB), 28-homobrassinolide (HB) and 24-epicastasteron (EC) were examined. Wheat cultivar Vasilisa was used. All tested BRs demonstrated inhibitory action on wheat root length. Dose-dependence of the effect showed complex bell-like shape. Low BR levels induced reactions similar to highest BR levels. Concentrations of 10–10 –10–9 mol/l caused maximal inhibition of root growth rate. At the same time, root diameter significantly increased after BR treatment, as did mean roots hair length. So the volume of the root did not change significantly while their architecture was dramatically modified by BRs. Manually performed root growth measurements were compared with tests by phenotyping and demonstrated that the designed phenotyping techniques provided very accurate data. The root architecture phenotyping also yielded accurate results. Overall, phenotyping method showed that BRs inhibited wheat root growth and induced increase of root diameter and length of root hairs.
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