Expression of GRP78 and GRP94 proteins in the human micro- and macrovascular endothelial cells under hypoxia

  • Tatyana O. Suhan Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus
  • Aleksandra S. Nevmerzhitskaya George Spaiher Institute of the Tumor Biotechnology and Experimental Therapy, P. Erlich Street, 42-44, 60596, Frankfurt-am-Main, Germany

Abstract

It was investigated the expression of stress activated chaperons GRP78, GRP94 in human microvascular and macrovascular endothelial cells in normoxic and hypoxic conditions of cultivations. The experiments were carried out on HUVEC (human umbilical vein endothelial cells), HDMEC (human dermal microvascular endothelial cells) and HMPEC (human microvascular placental endothelial cells). The proteins expression was estimated with immunoblotting. Densitometry of the immunoblotting results was done with «ImageJ» software. It was find out that in normoxic conditions pattern of GRP78 and GRP94 expression in HUVEC is similar to HDMEC and differs from HMPEC. Hypoxia changes expression of stress-induced proteins GRP78 and GRP94. Expression of GRP78 and GRP94 in microvascular endothelial cells (HDMEC, HMPEC) differs from expression in macrovascular endothelial cells (HUVEC). 

Author Biographies

Tatyana O. Suhan, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

PhD (biology); senior researcher at the scientific research laboratory of physiology at the department of human and animal physiology, faculty of biology

Aleksandra S. Nevmerzhitskaya, George Spaiher Institute of the Tumor Biotechnology and Experimental Therapy, P. Erlich Street, 42-44, 60596, Frankfurt-am-Main, Germany

research assistant

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Published
2017-12-16
Keywords: endothelial cells, hypoxia, heat shock proteins
How to Cite
Suhan, T. O., & Nevmerzhitskaya, A. S. (2017). Expression of GRP78 and GRP94 proteins in the human micro- and macrovascular endothelial cells under hypoxia. Experimental Biology and Biotechnology, 2, 17-24. Retrieved from https://journals.bsu.by/index.php/biology/article/view/2440
Issue
No 2 (2017): Journal of the Belarusian State University. Biology
Section
Physiology and Сell Biology

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