Moleculargenetic analysis of acdS gene of Pseudomonas putida B-37

  • Alesia A. Melnikava Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus
  • Sviatlana I. Leanovich Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus
  • Alena A. Khramtsova Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

Abstract

In current work PCR-screening of bacterial collection of genus Pseudomonas was made with the purpose to check the presence of acdS gene which is coding enzyme ACC deaminase. ACC deaminase can inhibit synthesis of stress ethylene that is harmful for the growth and development of plants. This gene was observed among three of five bacterial strains (P. putida B-37, P. putida B-28, P. putida KT 2442). Nucleotide sequence of the fragment corresponding to acdS gene of P. putida B-37 was taken and analyzed in silico. As a result, it was proven that there is acdS gene and it codes the protein ACC deaminase. Using of ACC deaminase will lead to increasing of stress ethylene level and negative influence on the plants of different environmental factors. 

Author Biographies

Alesia A. Melnikava, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

postgraduate student at the department of genetics, faculty of biology

Sviatlana I. Leanovich, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

student at the faculty of biology

Alena A. Khramtsova, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

PhD (biology); associate professor at the department of genetics, faculty of biology

References

  1. Glick B. R., Todorovic B., Czarny J., et al. Promotion of plant growth by bacterial ACC deaminase. Critical Rev. Plant Sci. 2007. Vol. 26, issue 5 – 6. P. 227–242. DOI: 10.1080/07352680701572966.
  2. Gontia-Mishra I., Sasidharan S., Tiwari S. Recent developments in use of 1-aminocyclopropane-1-carboxylate (ACC) deaminase for conferring tolerance to biotic and abiotic stress. Biotechnol. Lett. 2014. Vol. 36, issue 5. P. 889 –898. DOI: 10.1007/s10529014 -1458-9.
  3. Glick B., Pasternak J. Molecular biotechnology: principles and applications of recombinant DNA. Moscow, 2002 (in Russ.).
  4. Glick B. R. Modulation of plant ethylene levels by the bacterial enzyme ACC deaminase. FEMS Microbiol. Lett. 2005. Vol. 251, issue 1. P. 1–7. DOI: 10.1016/j.femsle.2005.07.030.
  5. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol. 1961. Vol. 3. P. 208 –218.
  6. Saravanakumar D. Rhizobacterial ACC deaminase in plant growth and stress amelioration. Bacteria in Agrobiology : stress mana gement. Heidelberg, 2012. P. 187–204. DOI: 10.1007/978-3-642-23465-1_9.
  7. Kamala-Kannan S., Lee K. J., Park S. M., et al. Characterization of ACC deaminase gene in Pseudomonas entomophila strain PS-PJH isolated from the rhizosphere soil. J. Basic Microbiol. 2010. Vol. 50. P. 200 –205.
  8. Kyte I., Doolittle R. F. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 1982. Vol. 157. P. 105–132.
  9. Guruprasad K., Bhasker Reddy B. V., Pandit M. Correlation between stability of a protein and its dipeptide composition: a novel approach for predicting in vivo stability of a protein from its primary sequence. Protein Eng., Design Selection. 1990. Vol. 4, No. 2. P. 155–161.
Published
2017-12-16
Keywords: ACC deaminase, ethylene, acdS gene, Pseudomonas
How to Cite
Melnikava, A. A., Leanovich, S. I., & Khramtsova, A. A. (2017). Moleculargenetic analysis of acdS gene of Pseudomonas putida B-37. Experimental Biology and Biotechnology, 2, 51-57. Retrieved from https://journals.bsu.by/index.php/biology/article/view/2445
Issue
No 2 (2017): Journal of the Belarusian State University. Biology
Section
Genetics, Microbiology and Molecular Biology

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