Comparative study of electro physiological characteristics of identified dopamine-containing (R.Pe.D.1) and serotonin-containing (L.Pe.D.1) neurons within CNS of mollusc Lymnaea stagnalis

  • Mohamed Shahrani Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus
  • Alexander V. Sidorov Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

Abstract

Microelectrode technique was use to determine parameters of spontaneous electrical activity of two interneurons within lung respiration (R.Pe.D.1) and locomotion (L.Pe.D.1) neuronal networks of Lymnaea stagnalis. Significant differences were detect for rest potential value and spike frequency: –58.1 ± 3.3 mV and 0.91 ± 0.14 Hz (R.Pe.D.1); – 49.0 ± 2.8 mV and 1.45 ± 0.12 Hz (L.Pe.D.1). For R.Pe.D.1, а voltage-current curve shifts to the right or to the left at the negative and positive currents respectively in comparison with L.Pe.D.1 were determined. The increase of single action potentials duration was observe in L.Pe.D.1 in comparison with R.Pe.D.1: 3.6-time for depolarization phase, 1.5-time for repolarization phase and 1.2-time for undershoot. Amplitudes of action potentials and all its parts (threshold, undershoot) did not vary significantly. We suggest that peculiarities of electrical properties of membrane underlie functional specificity of identified neurons within Lymnaea’s CNS.

Author Biographies

Mohamed Shahrani, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

postgraduate student at the department of human and animal physiology, faculty of biology

Alexander V. Sidorov, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

doctor of science (biology), docent; professor at the department of human and animal physiology, faculty of biology

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Published
2018-05-01
Keywords: rest potential, action potential, voltage-current curve, neuronal circuits, invertebrates
Supporting Agencies This work supported by State Program for Scientific Research «Fundamental and applied sciences for medicine» (task 1.08).
How to Cite
Shahrani, M., & Sidorov, A. V. (2018). Comparative study of electro physiological characteristics of identified dopamine-containing (R.Pe.D.1) and serotonin-containing (L.Pe.D.1) neurons within CNS of mollusc Lymnaea stagnalis. Experimental Biology and Biotechnology, 3, 3-9. Retrieved from https://journals.bsu.by/index.php/biology/article/view/2454
Issue
No 3 (2017): Journal of the Belarusian State University. Biology
Section
Physiology and Сell Biology

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