Comparative study of electro physiological characteristics of identified dopamine-containing (R.Pe.D.1) and serotonin-containing (L.Pe.D.1) neurons within CNS of mollusc Lymnaea stagnalis
Abstract
Microelectrode technique was use to determine parameters of spontaneous electrical activity of two interneurons within lung respiration (R.Pe.D.1) and locomotion (L.Pe.D.1) neuronal networks of Lymnaea stagnalis. Significant differences were detect for rest potential value and spike frequency: –58.1 ± 3.3 mV and 0.91 ± 0.14 Hz (R.Pe.D.1); – 49.0 ± 2.8 mV and 1.45 ± 0.12 Hz (L.Pe.D.1). For R.Pe.D.1, а voltage-current curve shifts to the right or to the left at the negative and positive currents respectively in comparison with L.Pe.D.1 were determined. The increase of single action potentials duration was observe in L.Pe.D.1 in comparison with R.Pe.D.1: 3.6-time for depolarization phase, 1.5-time for repolarization phase and 1.2-time for undershoot. Amplitudes of action potentials and all its parts (threshold, undershoot) did not vary significantly. We suggest that peculiarities of electrical properties of membrane underlie functional specificity of identified neurons within Lymnaea’s CNS.
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