Design of the general concept of regulated inactivation of the gene shikimatkinase for modification of the shikimat way in bacteria Bacillus subtilis
Abstract
In this work demonstrated the possibility of the transformation strains of B. subtilis used to create strains-producers of shikimic acid. For 6 strains of B. subtilis, with the ability to increased synthesis of tryptophan, produced amplicons region of the genome containing genes tmrB and aroI. RFLP-analysis of the received amplicons showed their genetic polymorphism, which may be the result of mutagenesis used when receiving strains-producers of tryptophan. Based on the PCR method designed a system for fast screening of transformants for the presence of recombination and genome rearrangements presence integrative structure comprising bacterial strains used chromosomes from transformants.
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