Preparation of gelatinum nanoparticles and investigation of their interaction with cultivated cells

  • Tatyana O. Suhan Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus
  • Tatyana G. Shutava Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, 36 F. Skaryny Street, Minsk 220141, Belarus
  • Anastasiya M. Ushenkina Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, 36 F. Skaryny Street, Minsk 220141, Belarus
  • Alla I. Potapovich Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus
  • Vladimir A. Kostyuk Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

Abstract

Experimental data showing that gelatin nanoparticles may be used as a means of delivery of pharmacologically active substances into cultured human normal and cancer cells are presented in the paper. It was found that the incubation of the encapsulated dye Nile red with the cells of the human breast adenocarcinoma MDA-MB-231 and normal fibroblasts resulted to the increase of the dye content in the cells with the increasing incubation time and reached a maximum after 24 h, with the accumulation of dye in cancer cells much faster than in fibroblasts. It was found that the inclusion of quercetin in gelatin nanoparticles led to a significant increase in cytotoxic effect of this polyphenol against cancer cells.

Author Biographies

Tatyana O. Suhan, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

PhD (biology); senior researcher at the scientific research laboratory of physiology at the department of human and animal physiology, faculty of biology

Tatyana G. Shutava, Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, 36 F. Skaryny Street, Minsk 220141, Belarus

PhD (chemistry); leading researcher at the laboratory of biopolymer encapsulated structures

Anastasiya M. Ushenkina, Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, 36 F. Skaryny Street, Minsk 220141, Belarus

laboratory assistant at the laboratory of biopolymer encapsulated structures

Alla I. Potapovich, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

PhD (biology); leading researcher at the scien tific research laboratory of physiology at the department of human and animal physiology, faculty of biology

Vladimir A. Kostyuk, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

doctor of science (chemistry), docent; head of the scientific research laboratory of physiology at the department of human and animal physiology, faculty of biology

References

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Published
2018-05-03
Keywords: gelatin nanoparticles, fibroblasts, cell line MDA-MB-231, quercetin, Nile red
How to Cite
Suhan, T. O., Shutava, T. G., Ushenkina, A. M., Potapovich, A. I., & Kostyuk, V. A. (2018). Preparation of gelatinum nanoparticles and investigation of their interaction with cultivated cells. Experimental Biology and Biotechnology, 1, 13-19. Retrieved from https://journals.bsu.by/index.php/biology/article/view/2482
Issue
No 1 (2018): Journal of the Belarusian State University. Biology
Section
Physiology and Сell Biology

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