Time-course and amplitude characteristics of action potentials of identified Lymnaea stagnalis neurons under the hydrogen peroxide impact

  • Alexander V. Sidorov Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

Abstract

Microelectrode technique was used to determine the parameters of spontaneous spikes of two identified dopamine-containing (R.Pe.D.1) and serotonin-containing (L.Pe.D.1) neurons under the action of hydrogen peroxide. H2O2 (1 mmol/l) bath application on the surface of isolated CNS did not results in significant changes of time-course and amplitude characteristics of the neurons under study. Strengthening of H2O2 concentration (100 mmol/l) evoke the increase of action potential duration (de- and repolarization phases both) and decrease of spike, undershoot and threshold amplitudes in R.Pe.D.1. For L.Pe.D.1, the same trend was observing only for depolarisation phase duration, spike and threshold amplitudes. It is assume that peculiarities of electrical properties of membranes are due to the differences in the sensitivity of Na+- and K+- conductivity to reactive oxygen species. That state could underlie functional specificity of identified neurons within CNS of Lymnaea stagnalis and the diversity of neuronal response to the nervous tissue redox disbalance. 

Author Biography

Alexander V. Sidorov, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

doctor of science (biology), docent; professor at the department of human and animal physiology, faculty of biology

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Published
2018-05-03
Keywords: rest potential, spike, reactive oxygen species, mollusc
Supporting Agencies This work supported by state programs for scientific Research «Convergence» (task 3.3.03.4) and «Fundamental and applied sciences for medicine» (task 1.08).
How to Cite
Sidorov, A. V. (2018). Time-course and amplitude characteristics of action potentials of identified Lymnaea stagnalis neurons under the hydrogen peroxide impact. Experimental Biology and Biotechnology, 1, 31-37. Retrieved from https://journals.bsu.by/index.php/biology/article/view/2484
Issue
No 1 (2018): Journal of the Belarusian State University. Biology
Section
Physiology and Сell Biology

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