Antiradical activity, antimutagenic and antigenic properties of enzymatic bovine colostrum hydro lysates

  • Tatsiana M. Halavach Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus
  • Ekaterina I. Tarun International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus
  • Natalia V. Dudchik Scientific Practical Centre of Hygiene, 8 Akademičnaja Street, Minsk 220012, Belarus
  • Roman V. Romanovich Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus
  • Ilona A. Bubra Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus
  • Vladimir P. Kurchenko Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

Abstract

A comparative analysis of protein and peptide profile, biological active properties of hydrolysed bovine colostrum obtained using various endopeptidases (alcalase and neutrase) was performed. It was found that hydrolysis with alcalase and ultrafiltration led to increase in antioxidant activity of the peptide fraction by 7.1 times whereas using neutrase by 3.5 times. Maximum antimutagenic effect was shown for experiment with alcalase, that attained 10.0 –29.6 % for strain Salmonella typhimurium TA 98 and 12.5–32.4 % for strain TA 100. Ultrafiltrate of hydrolysate using alcalase is enriched with specific short-chain peptides which determine its relatively high antimutagenic and antiradical properties. According to SDS-electrophoresis and reaction of immunoprecipitation alcalase effectively cleaves β-lactoglobulin, that provides a hydrolysate producing with low allergen potential. Neutrase cleaves this protein substrate partially. Using immunochemical analysis lactoferrin was detected in all samples of hydrolysed colostrum. In accordance with experimental data the raise in antioxidant and antimutagenic potential and also the decline in antigenic properties of hydrolysed colostrum are due to an increase in depth of proteolysis.

Author Biographies

Tatsiana M. Halavach, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

PhD (biology); senior researcher at the laboratory of applied biology, faculty of biology

Ekaterina I. Tarun, International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus

PhD (chemistry), docent; associate professor at the department of environmental chemistry and biochemistry, faculty of environmental medicine

Natalia V. Dudchik, Scientific Practical Centre of Hygiene, 8 Akademičnaja Street, Minsk 220012, Belarus

doctor of science (biology), docent; head of the laboratory of microbiology

Roman V. Romanovich, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

student at the faculty of biology

Ilona A. Bubra, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

student at the faculty of biology

Vladimir P. Kurchenko, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

PhD (biology), docent; head of the laboratory of applied biology, faculty of biology

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Published
2018-05-03
Keywords: bovine colostrum, enzymatic hydrolysis, alcalase, neutrase, protein and peptide profile, antioxidant properties, antimutagenic activity, antigenic properties, β-lactoglobulin, lactoferrin
How to Cite
Halavach, T. M., Tarun, E. I., Dudchik, N. V., Romanovich, R. V., Bubra, I. A., & Kurchenko, V. P. (2018). Antiradical activity, antimutagenic and antigenic properties of enzymatic bovine colostrum hydro lysates. Experimental Biology and Biotechnology, 1, 50-59. Retrieved from https://journals.bsu.by/index.php/biology/article/view/2486
Issue
No 1 (2018): Journal of the Belarusian State University. Biology
Section
Biochemistry, Biotechnology and Microbiology

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