The antifungal activity of ray fungum against the fusarium wilt causal agent of tomato
Abstract
The antifungal activity of genus Streptomyces strains (from collection of department of microbiology, Belarusian State University) against the fusarium wilt causal agent of tomato micromycetes Fusarium oxysporum f. lycopersici (Sacc.) Snyder and Hansen were studied in laboratory model experiments. Dynamic of micromycete colonies growth, morphology, color and fruiting intensity were analyzed. The screening of tested strains of genus Streptomyces for their anti-wilt activity has showed that the strain 10 influences the inhibiting (more than 60 %) effect on all fusaruim isolates: high-pathogenic (Fol 1), middle-pathogenic (Т 2) and low-pathogenic (Т 11). Their colonies growth were blocked from the 4th day of joint cultivation. The strain 11 has been an antagonist for the isolates Fol 1 and T 2, and the strain 20 – for the isolate T 11. Strains 18 and 35 demonstrate no antifungal activity to all F.oxysporum f. lycopercisi isolates under study.
References
- Poliksenova VD. [Fusarium wilt of tomato]. Zashchita rastenii. 1987;6:51–52. Russian.
- Piskun SG, Poliksenova VD, Anokhina VS. Intraspecific differentiation of the causative agent of fusarium wilting of tomatoes. Vestnik BGU. Seriya 2. Khimiya. Biologiya. Geografiya. 2002;3:36 – 41. Russian.
- Piskun SG, Poliksenova VD, Anokhina VS. Phitotoxic activity by fusarium wilt of tomato causal agent. Vestnik BGU. Seriya 2. Khimiya. Biologiya. Geografiya. 2003;2:87– 89. Russian.
- Poliksenova VD. Mikozy tomata: vozbuditeli zabolevanii, ustoichivost’ rastenii [Tomato mycosis: pathogens, plants resistance]. Minsk: Belarusian State University; 2008. 159 p. Russian.
- Sokolov MS, Monastirskiy ОА, Pokushova YeA. Ekologizatsiya zashchity rastenii [Ecologization of plant protection]. Pushchino: ONTI PNTs RAN; 1994. 462 p. Russian.
- Tarkka M, Hampp R. Secondary metabolites of soil streptomycetes in biotic interaction. In: Karlovsky P, editor. Secondary Metabolites in Soil Ecology. Volume 14. Soil biology. Berlin: Springer; 2008. р. 107–126.
- Hoster F, Schmitz JE, Daniel R. Enrichment of chitinolytic microorganisms: isolation and characterization of a chitinase exhibiting antifungal activity against phytopathogenic fungi from a novel Streptomyces strain. Applied Microbiology and Biotechnology. 2005;66(4):434 – 442. DOI: 10.1007/s00253-004-1664-9.
- Chater KF, Biro S, Lee KJ, Painer T, Schrempf H. The complex extracellular biology of streptomycetes. FEMS Microbiology Reviews. 2010;34(2):171–198. DOI: 10.1111/j.1574-6976.2009.00206.x.
- Strap JL. Actinobacteria – plant interactions: A boon to agriculture. In: Maheshwari DK, editor. Bacteria in agrobiology: plant growth responses. Berlin: Springer; 2011. p. 285–307.
- Goodfellow M, Simpson KE. Ecology of streptomycetes. Frontiers in Applied Microbiology. 1987;2:97–125.
- Zvyagintsev DG, Zenova GM. Ekologiya aktinomitsetov [Ecology of actinomycetes]. Moscow: GEOS; 2001. 257 p. Russian.
- Shirokikh IG. [Antifungal potential of actinomycetes in the rhizosphere of barley on sod-podzol soils]. Pochvovedenie. 2003; 4:458 – 464. Russian.
- Vinogradova KA, Sharkova TS, Aleksandrova AB, Kozhevin PA. [Analysis of interpopulation interactions of soil fungi and actinomycetes]. Mikologiya i fitopatologiya. 2005;39(3):28 – 40. Russian.
- Novikova II, Boykova IV, Shenin YuD. [Biological features and component composition of the active complex of the strain Streptomyces chrysomallus P-21 an antagonist of phytopathogenic fungi]. Vestnik zashchity rastenii. 2006;3:13–21. Russian.
- Omarova EO, Zenova VK, Orleanskiy VK, Karpov GA, Zhegallo EA. [Environmental features of the interaction of cyanobacteriae and streptomycetes as components of algobacterial associations]. In: Griby i vodorosli v biotsenozakh. Materialy mezhdunarodnoi konferentsii, posvyashchennoi 75-letiyu biologicheskogo fakul’teta MGU im. M. V. Lomonosova; 31 yanvarya – 3 fevralya 2006 g.; Moskva, Rossiya [Fungi and algae in biocenoses. Materials of the international conference dedicated to the 75th anniversary of the faculty of biology of Lomonosov Moscow State University; 2006 January 31 – February 3; Moscow, Russia. Moscow: MAKS Press; 2006. p. 116 –117. Russian.
- Domracheva LI, Shirokikh IG, Fokina AI. Cyanobacteria andactinomycetes influence againstfusariumspecies in soil and rhizosphere. Mikologiya i fitopatologiya. 2009;43(2):157–165. Russian.
- Belyavskaya LA, Kopylov EP, Shakhovnina EA, Kozyritskaya VE, Iutinskaya GA. [Streptomycetes are promising producers of biopesticides]. In: D’yakov YuT, editor. Sovremennaya mikologiya v Rossii. Tom 3. Tezisy dokladov 3-go s’ezda mikologov Rossii [Modern mycology in Russia. Volume 3. Abstracts of the 3rd Congress of mycologists of Russia]. Moscow: National Academy of Mycology; 2012. p. 332–333. Russian.
- Shirokikh IG, Ryabova OV, Kharina AB, Korjakovtseva LA, Shirokikh AA. Impact of Streptomyces hygroscopicus A-4 strain on microfungal complexes in the wheat rhizosphere. Mikologiya i fitopatologiya. 2013;47(6):410 – 416. Russian.
- Burtseva SA, Byrsa MN, Berezyuk YuN, Vasilchuk AV. [Ability to inhibit the growth of phytopathogenic fungi in streptomycetes of Moldavian soils]. In: Dʼyakov YuT, Sergeev YuV, editors. Sovremennaya mikologiya v Rossii. Tom 7. Tezisy dokladov 4-go s’ezda mikologov Rossii [Modern mycology in Russia. Volume 7. Abstracts of the 4th Congress of mycologists of Russia]. Moscow: National Academy of Mycology; 2017. p. 20 –22. Russian.
- Ratkevich EB, Sidorova SG. [Antifungal activity of fungi of the genus Trichoderma Pers.: Fr. and actinomycetes against the causative agent of tomato fusarium wilt. In: Smolich II, Demidchik VV, Padutov VE, editors. Kletochnaya biologiya i biotekhnologiya rastenii. Tezisy dokladov II Mezhdunarodnoi nauchno-prakticheskoi konferentsii; 28–31 maya 2018 g.; Minsk, Belarus’ [Cell biology and plant biotechnology. Abstracts of the II International scientific and practical conference; 2018 May 28–31; Minsk, Belarus]. Minsk: BSU Publishing Center; 2018. p. 73–74. Russian.
- Dudka IA, Vasser SP, Ellanskaya IA, Koval’ EZ, Gorbik LT, Nikol’skaya EA, et al. Metody eksperimental’noi mikologii [Experimental mycology methods]. Kiev: Naukova dumka; 1982. 52 p. Russian.
- Poliksenova VD, Khramtsov AK, Piskun SG. Metodicheskie ukazaniya k zanyatiyam spetspraktikuma po razdelu «Mikolo giya. Metody eksperimental’nogo izucheniya mikroskopicheskikh gribov» dlya studentov 4-go kursa dnevnogo otdeleniya spetsial’nosti G 31 01 01 «Biologiya» [Methodical instructions for special workshop in the section «Mycology. Methods of experimental study of microscopic mushrooms» for 4th year students of the full-time department of speciality G 31 01 01 «Biologiya»]. Minsk: Belarusian State University; 2004. 38 p. Russian.
- Strunnikova OK, Shakhnazarova VYu, Vishnevskaya NA. Development and relationship of the phytopathogenic fungus Fusarium culmorum and the antagonistic bacterium Pseudomonas fluorescens in soil, rhizosphere and on the roots of barley. In: Fitosanitarnoe ozdorovlenie ekosistem. Materialy II Vserossiiskogo s’ezda po zashchite rastenii; 10 dekabrya 2005 g.; Sankt-Peterburg, Rossiya [Phytosanitary rehabilitation of ecosystems. Materials of the II All-Russian Congress on plant protection; 2005 December 10; Saint Petersburg, Russia]. Saint Petersburg: All-Russian Institute of Plant Protection; 2005. p. 193–194. Russian.
- Zenova GM. Pochvennye aktinomitsety [Soil actinomycetes]. Moscow: Lomonosov Moscow State University; 1992. 78 p. Russian.
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