Receptor-mediated endocytosis is an important way for gelatin nano-particles penetration into cells

  • Alla I. Potapovich Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Tatyana O. Suhan Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Tatsiana G. Shutava Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, 36 F. Skaryny Street, Minsk 220141, Belarus
  • Vladimir A. Kostyuk Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus https://orcid.org/0000-0002-1246-4131

Abstract

Experimental data detailing the possibilities of using gelatin nanoparticles obtained by the two-stage desolvation method without the use of surfactants for delivering pharmacologically active substances to cultured normal and human cancer cells are presented. It was shown that clathrin-dependent endocytosis is the main route of entry of such nanoparticles into normal human fibroblasts and cancer cells of the MDA-MB-231 line. Due to this type of endocytosis, more than 50 % of gelatin nanoparticles enter the cells. It was shown that in the process of clathrin-dependent endocytosis, gelatin nanoparticles specifically bind to collagen receptors.

Author Biographies

Alla I. Potapovich, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

PhD (biology); leading researcher at the research laboratory of physiology at the department of human and
animal physiology, faculty of biology

Tatyana O. Suhan, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

PhD (biology); senior researcher at the research laboratory of physiology at the department of human and animal physiology, faculty of biology

Tatsiana G. Shutava, Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, 36 F. Skaryny Street, Minsk 220141, Belarus

PhD (chemistry); leading researcher at the laboratory of biopolymer encapsulated structures

Vladimir A. Kostyuk, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

doctor of science (chemistry), docent; head of the research laboratory of physiology at the department of human and animal physiology, faculty of biology

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Published
2020-02-18
Keywords: endocytosis, gelatin nanoparticles, fibroblasts, cell line MDA-MB-231, Nile red, gelatin, сhlorpromazine
How to Cite
Potapovich, A. I., Suhan, T. O., Shutava, T. G., & Kostyuk, V. A. (2020). Receptor-mediated endocytosis is an important way for gelatin nano-particles penetration into cells. Experimental Biology and Biotechnology, 1, 3-10. https://doi.org/10.33581/2521-1722-2020-1-3-10