Receptor-mediated endocytosis is an important way for gelatin nano-particles penetration into cells

  • Alla I. Potapovich Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Tatyana O. Suhan Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Tatsiana G. Shutava Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, 36 F. Skaryny Street, Minsk 220141, Belarus
  • Vladimir A. Kostyuk Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus https://orcid.org/0000-0002-1246-4131
DOI: https://doi.org/10.33581/2521-1722-2020-1-3-10

Abstract

Experimental data detailing the possibilities of using gelatin nanoparticles obtained by the two-stage desolvation method without the use of surfactants for delivering pharmacologically active substances to cultured normal and human cancer cells are presented. It was shown that clathrin-dependent endocytosis is the main route of entry of such nanoparticles into normal human fibroblasts and cancer cells of the MDA-MB-231 line. Due to this type of endocytosis, more than 50 % of gelatin nanoparticles enter the cells. It was shown that in the process of clathrin-dependent endocytosis, gelatin nanoparticles specifically bind to collagen receptors.

Author Biographies

Alla I. Potapovich, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

PhD (biology); leading researcher at the research laboratory of physiology at the department of human and
animal physiology, faculty of biology

Tatyana O. Suhan, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

PhD (biology); senior researcher at the research laboratory of physiology at the department of human and animal physiology, faculty of biology

Tatsiana G. Shutava, Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, 36 F. Skaryny Street, Minsk 220141, Belarus

PhD (chemistry); leading researcher at the laboratory of biopolymer encapsulated structures

Vladimir A. Kostyuk, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

doctor of science (chemistry), docent; head of the research laboratory of physiology at the department of human and animal physiology, faculty of biology

References

  1. Sahoo SK, Labhasetwar V. Nanotech approaches to drug delivery and imaging. Drug Discovery Today. 2003;8(24):1112–1120. DOI: 10.1016/S1359-6446(03)02903-9.
  2. Bareford LM, Swaan PW. Endocytic mechanisms for targeted drug delivery. Advanced Drug Delivery Reviews. 2007;59(8):748 –758. DOI: 10.1016/j.addr.2007.06.008.
  3. Akinc A, Battaglia G. Exploiting endocytosis for nanomedicines. Cold Spring Harbor Perspectives in Biology [Internet]. 2013 [cited 2019 July 1];5(11):a016980. Available from: https://cshperspectives.cshlp.org/content/5/11/a016980.full. DOI: 10.1101/cshperspect.a016980.
  4. Birge RB, Ucker DS. Innate apoptotic immunity: the calming touch of death. Cell Death and Differentiation. 2008;15(7):1096 –1102.
  5. Wang J, Byrne JD, Napier ME, DeSimone JM. More effective nanomedicines through particle design. Small. 2011;7(14):1919 –1931. DOI: 10.1002/smll.201100442.
  6. Rappoport JZ. Focusing on clathrin-mediated endocytosis. Biochemical Journal. 2008;412(3):415– 423. DOI: 10.1042/BJ20080474.
  7. Zhang S, Li J, Lykotrafitis G, Bao G, Suresh S. Size-dependent endocytosis of nanoparticles. Advanced Materials. 2009;21(4):419 – 424. DOI: 10.1002/adma.200801393.
  8. Suhan TO, Shutova TG, Ushenkina AM, Potapovich AI, Kostyuk VA. Obtaining gelatin nanoparticles and study their interaction with cultured cells. Journal of Belarusian State University. Biology. 2018;1:13–19. Russian.
  9. Coester CJ, Langer K, Von Briesen H, Kreuter J. Gelatin nanoparticles by two step desolvation a new preparation method, surface modifications and cell uptake. Journal of Microencapsulation. 2000;17(2):187–193. DOI: 10.1080/026520400288427.
  10. Sorensen KK, Simon-Santamaria J, McCuskey RS, Smedsrоd B. Liver sinusoidal endothelial cells. Comprehensive Physiology. 2015;5(4):1751–1774. DOI: 10.1002/cphy.c140078.
  11. Smedsrod B, Melkko J, Risteli L, Risteli J. Circulating C‐terminal propeptide of type I procollagen is cleared mainly via the mannose receptor in liver endothelial cells. Biochemical Journal. 1990;271(2):345–350. DOI: 10.1042/bj2710345.
  12. Lu ML, McCarron RJ, Jacobson BS. Initiation of HeLa cell adhesion to collagen is dependent upon collagen receptor upregulation, segregation to the basal plasma membrane, clustering and binding to the cytoskeleton. Journal of Cell Science. 1992;101(4):873– 883.
  13. Vina-Vilaseca A, Bender-Sigel J, Sorkina T, Closs EI, Sorkin A. Protein kinase C-dependent ubiquitination and clathrin-mediated endocytosis of the cationic amino acid transporter CAT-1. Journal of Biological Chemistry. 2011;286(10):8697– 8706. DOI: 10.1074/jbc.M110.186858.
  14. Kovács T, Kárász A, Szöllősi J, Nagy P. The density of GM1-enriched lipid rafts correlates inversely with the efficiency of transfection mediated by cationic liposomes. Cytometry. Part A. 2009;75A(8):650 – 657. DOI: 10.1002/cyto.a.20756.
  15. Harush-Frenkel O, Rozentur E, Benita S, Altschuler Y. Surface charge of nanoparticles determines their endocytic and transcytotic pathway in polarized MDCK cells. Biomacromolecules. 2008;9(2):435– 443. DOI: 10.1021/bm700535p.
  16. Vighi E, Leo E. Studying the in vitro behavior of cationic solid lipid nanoparticles as a nonviral vector. Nanomedicine. 2012;7(1):9 –12. DOI: 10.2217/nnm.11.168.
Published
2020-02-18
Keywords: endocytosis, gelatin nanoparticles, fibroblasts, cell line MDA-MB-231, Nile red, gelatin, сhlorpromazine
How to Cite
Potapovich, A. I., Suhan, T. O., Shutava, T. G., & Kostyuk, V. A. (2020). Receptor-mediated endocytosis is an important way for gelatin nano-particles penetration into cells. Experimental Biology and Biotechnology, 1, 3-10. https://doi.org/10.33581/2521-1722-2020-1-3-10
Issue
No 1 (2020): Journal of the Belarusian State University. Biology
Section
Cell Biology and Physiology

Copyright (c) 2020 Journal of the Belarusian State University. Biology

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

The authors who are published in this journal agree to the following:

  1. The authors retain copyright on the work and provide the journal with the right of first publication of the work on condition of license Creative Commons Attribution-NonCommercial. 4.0 International (CC BY-NC 4.0).
  2. The authors retain the right to enter into certain contractual agreements relating to the non-exclusive distribution of the published version of the work (e.g. post it on the institutional repository, publication in the book), with the reference to its original publication in this journal.
  3. The authors have the right to post their work on the Internet (e.g. on the institutional store or personal website) prior to and during the review process, conducted by the journal, as this may lead to a productive discussion and a large number of references to this work. (See The Effect of Open Access.)