Altered production of membrane-associated proteins, providing iodine accumulation by thyroid gland, after exposure to endocrine disruptor DDT

  • Sergey S. Obernikhin Research Institute of Human Morphology, 3 Tsyurupy Street, Moscow 117418, Russia
  • Nataliya V. Yaglova Research Institute of Human Morphology, 3 Tsyurupy Street, Moscow 117418, Russia
  • Svetlana V. Nazimova Research Institute of Human Morphology, 3 Tsyurupy Street, Moscow 117418, Russia
  • Valentin V. Yaglov Research Institute of Human Morphology, 3 Tsyurupy Street, Moscow 117418, Russia
DOI: https://doi.org/10.33581/2521-1722-2020-3-3-12

Abstract

Exposure to endocrine disruptors has a sufficient impact in rise of thyroid disorders. Iodine accumulation by the thyroid is provided by sodium iodide symporter, a glycoprotein, located in the basolateral membrane of thyroid cells. Therefore, sodium iodide symporter might be a putative target for endocrine disruptors. Objective of the research – investigation of sodium iodide symporter production after long-term exposure to endocrine disruptor dichlorodiphenyltrichloroethane (DDT) in different periods on postnatal development. Newly borne and 7-week old male Wistar rats were exposed to low doses of DDT during 6 and 10 weeks. Expression of sodium iodide symporter in thyroid cells was evaluated by immunohistochemistry. Quantification of sodium iodide symporter, thyroid hormones (T3 and T4) and thyroid stimulating hormone (TSH) in serum was performed by enzyme-linked immunosorbent assay. The investigation also included morphological examination of thyroid slides and morphometry. Adult rats after 6 weeks of exposure showed diminished level of total T4 and increase in T3 production, associated with significantly lowered TSH and sodium iodide symporter serum concentration. Rats, exposed to endocrine disruptor since birth, demonstrated slight decrease in T4 and sodium iodide symporter. Reduced level of free T3 and elevated serum TSH were found in adult rats after 10 weeks of exposure. Exceeded level of serum sodium iodide symporter was revealed. It was provided by increased number of thyroid cells, producing this protein, due to formation of numerous microfollicles in the thyroid gland. Young rats after 10 weeks of exposure demonstrated diminished total and free T3 levels and elevated TSH. No up-regulation of sodium iodide symporter expression was found. Morphological examination revealed enlarged follicles and no microfollicular rearrangement of thyroid parenchyma. Considered that low dose exposure to endocrine disruptor DDT irreversibly depresses expression of membrane glycoprotein sodium iodide symporter, which provides iodine uptake by the thyroid gland. After-birth exposure impairs compensatory activation of proliferation and increase in number of sodium iodide symporter-producing cells.

Author Biographies

Sergey S. Obernikhin, Research Institute of Human Morphology, 3 Tsyurupy Street, Moscow 117418, Russia

doctor of science (medicine); senior researcher at the laboratory of endocrine system development

Nataliya V. Yaglova, Research Institute of Human Morphology, 3 Tsyurupy Street, Moscow 117418, Russia

doctor of science (medicine); head of the laboratory of endocrine system development

Svetlana V. Nazimova, Research Institute of Human Morphology, 3 Tsyurupy Street, Moscow 117418, Russia

PhD (medicine); senior researcher at the laboratory of endocrine system development

Valentin V. Yaglov, Research Institute of Human Morphology, 3 Tsyurupy Street, Moscow 117418, Russia

doctor of science (medicine), full professor; chief researcher at the laboratory of endocrine system development

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Published
2020-12-31
Keywords: thyroid gland, sodium iodide symporter, thyroid hormones, morphology, endocrine disruptor, DDT
Supporting Agencies The research was financially supported by the Ministry of Science and Higher Education of the Russian Federation (registration number АААА-А17-117013050048-6)
How to Cite
Obernikhin, S. S., Yaglova, N. V., Nazimova, S. V., & Yaglov, V. V. (2020). Altered production of membrane-associated proteins, providing iodine accumulation by thyroid gland, after exposure to endocrine disruptor DDT. Experimental Biology and Biotechnology, 3, 3-12. https://doi.org/10.33581/2521-1722-2020-3-3-12
Issue
No 3 (2020): Journal of the Belarusian State University. Biology
Section
Cell Biology and Physiology

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