Antibacterial properties of silver nanoparticles and their membranotrophic action

  • Lilit S. Gabrielyan Yerevan State University, 1 Alex Manoukian Street, Yerevan 0025, Armenia; Russian-Armenian University, 123 Hovsep Emin Street, Yerevan 0051, Armenia
  • Armen A. Trchounian Yerevan State University, 1 Alex Manoukian Street, Yerevan 0025, Armenia; Russian-Armenian University, 123 Hovsep Emin Street, Yerevan 0051, Armenia
DOI: https://doi.org/10.33581/2521-1722-2020-3-64-71

Abstract

Nowadays, metal nanoparticles (NPs) can be considered as alternative agents for overcoming antibiotic resistance. The effect of silver NPs on gram-positive (Enterococcus hirae ATCC9790, Staphylococcus aureus MDC5233) and gram-negative (Escherichia coli K-12, Salmonella typhimurium MDC1759) bacteria have been investigated in this work. Silver NPs demonstrated antibacterial activity against the bacteria used, expressed in the decrease of the specific growth rate and the number of colony forming units. Moreover, the bactericidal effect of silver NPs on gram-positive bacteria was more pronounced than on gram-negative bacteria. In order to find out the mechanisms of NPs’ effects, the change of the redox potential, the hydrogen (H2) production ability, and the protons flux across the bacterial membrane have been also studied. The addition of NPs led to inhibition of H2 yield and to change of the energy-dependent proton flux through Fo F1-ATPase, indicating the silver NPs effect on the activity of membrane-bound enzymes. The data obtained point out that silver NPs show a pronounced antibacterial effect against the studied bacteria and can be used in biomedicine.

Author Biographies

Lilit S. Gabrielyan, Yerevan State University, 1 Alex Manoukian Street, Yerevan 0025, Armenia; Russian-Armenian University, 123 Hovsep Emin Street, Yerevan 0051, Armenia

PhD (biology), docent; associate professor at the department of biochemistry, microbiology and biotechnology, faculty of biology, Yerevan State University, and associate professor at the department of medical biochemistry and biotechnology, Institute of Biomedicine and Pharmacy, Russian-Armenian University.

Armen A. Trchounian, Yerevan State University, 1 Alex Manoukian Street, Yerevan 0025, Armenia; Russian-Armenian University, 123 Hovsep Emin Street, Yerevan 0051, Armenia

corresponding member of the National Academy of Sciences of the Republic of Armenia, honored scientist of the Republic of Armenia, doctor of science (biology), full professor; head of the department of biochemistry, microbiology and biotechnology, faculty of biology, Yerevan State University , and professor at the department of medical biochemistry and biotechnology, Institute of Biomedicine and Pharmacy, Russian-Armenian University

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Published
2021-01-15
Keywords: silver nanoparticles, bacterial growth, redox potential, energy-dependent proton flux, hydrogen production
Supporting Agencies This work was supported by basic support from Russian-Armenian University provided by the Ministry of Education and Science of the Russian Federation.
How to Cite
Gabrielyan, L. S., & Trchounian, A. A. (2021). Antibacterial properties of silver nanoparticles and their membranotrophic action. Experimental Biology and Biotechnology, 3, 64-71. https://doi.org/10.33581/2521-1722-2020-3-64-71
Issue
No 3 (2020): Journal of the Belarusian State University. Biology
Section
Biotechnology and Microbiology

Copyright (c) 2021 Journal of the Belarusian State University. Biology

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