Influence of polyelectrolytes on increasing sensitivity of an immunofluorescent analysis based on plasmon silver nanoparticles

  • Irina V. Koktysh International Sakharov Environmental Institute, Belarusian State University, 23 Daŭhabrodskaja Street, 1 building, Minsk 220070, Belarus
  • Yanina I. Mel’nikova International Sakharov Environmental Institute, Belarusian State University, 23 Daŭhabrodskaja Street, 1 building, Minsk 220070, Belarus
  • Olga S. Kulakovich B. I. Stepanov Institute of Physics, National Academy of Sciences of Belarus, 68 Niezaliežnasci Avenue, 2 building, Minsk 220072, Belarus
  • Andrei A. Ramanenka B. I. Stepanov Institute of Physics, National Academy of Sciences of Belarus, 68 Niezaliežnasci Avenue, 2 building, Minsk 220072, Belarus
  • Sergey A. Maskevich International Sakharov Environmental Institute, Belarusian State University, 23 Daŭhabrodskaja Street, 1 building, Minsk 220070, Belarus
DOI: https://doi.org/10.33581/2521-1722-2020-3-72-80

Abstract

It was studied the dependence of the interaction of the components of the immunofluorescence test system for the quantitative determination of prostate specific antigen based on plasmon silver nanoparticles on the physicochemical nature of various polyelectrolytes used to coat films of silver nanoparticles. It has been shown that the use of a weakly charged polycationic polyelectrolyte poly-L-lysine can increase the antigenic binding of the test system by 2.34 times, and the use of a highly charged polycationic polyelectrolyte polydiallyldimethylammonium chloride increases the binding affinity of prostatic specific antigen by 5 times. When developing various immunochemical test systems using films of silver nanoparticles, an important parameter is the choice of a polyelectrolyte for coating a silver nanolayer, since the physicochemical and electrostatic properties of the polyelectrolyte can significantly affect both the sorption capacity of the solid phase and the conformational state functional activity of immobilized protein molecules. Both specificity and sensitivity of the immunochemical test system, as well as the minimum possible detectable concentration of bioanalyte, largely depend on these parameters.

Author Biographies

Irina V. Koktysh, International Sakharov Environmental Institute, Belarusian State University, 23 Daŭhabrodskaja Street, 1 building, Minsk 220070, Belarus

PhD (biology); head of the laboratory of environmental biotechnology

Yanina I. Mel’nikova, International Sakharov Environmental Institute, Belarusian State University, 23 Daŭhabrodskaja Street, 1 building, Minsk 220070, Belarus

senior lecturer at the department of immunology, faculty of environmental medicine

Olga S. Kulakovich, B. I. Stepanov Institute of Physics, National Academy of Sciences of Belarus, 68 Niezaliežnasci Avenue, 2 building, Minsk 220072, Belarus

PhD (chemistry); deputy head of the Nanophotonics Center

Andrei A. Ramanenka, B. I. Stepanov Institute of Physics, National Academy of Sciences of Belarus, 68 Niezaliežnasci Avenue, 2 building, Minsk 220072, Belarus

researcher at the Nanophotonics Center

Sergey A. Maskevich, International Sakharov Environmental Institute, Belarusian State University, 23 Daŭhabrodskaja Street, 1 building, Minsk 220070, Belarus

doctor of science (physics and mathematics), full professor; director

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Published
2021-01-15
Keywords: fluorescence immunological analysis, polyelectrolytes, poly-L-lysine, polydiallyldimethylammonium chloride, fluorescein isothiocyanate, fluorescence, silver nanoparticles, plasmonics, prostate-specific antigen
Supporting Agencies The study was conducted within agreement of B. I. Stepanov Institute of Physics, National Academy of Sciences of Belarus, with the Belarusian Republican Foundation for Fundamental Research No. F20PTI-004 dated 2020 May 4 (the project «Investigation of the mechanism of plasmon accumulation of photoluminescence in anisotropic nanostructures»).
How to Cite
Koktysh, I. V., Mel’nikova, Y. I., Kulakovich, O. S., Ramanenka, A. A., & Maskevich, S. A. (2021). Influence of polyelectrolytes on increasing sensitivity of an immunofluorescent analysis based on plasmon silver nanoparticles. Experimental Biology and Biotechnology, 3, 72-80. https://doi.org/10.33581/2521-1722-2020-3-72-80
Issue
No 3 (2020): Journal of the Belarusian State University. Biology
Section
Biotechnology and Microbiology

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